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Literature summary extracted from

  • Hoch, S.O.
    Isolation and characterization of two tryptophan biosynthetic enzymes, indoleglycerol phosphate synthase and phosphoribosyl anthranilate isomerase, from Bacillus subtilis (1979), J. Bacteriol., 139, 362-368.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
4.1.1.48 optimal stabilization by 0.8 M sucrose Bacillus subtilis
5.3.1.24 10-15% v/v glycerol stabilizes Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.48 23500
-
 1 * 23500, SDS-PAGE Bacillus subtilis
4.1.1.48 31000
-
 gel filtration Bacillus subtilis
5.3.1.24 21800
-
 1 * 21800, SDS-PAGE Bacillus subtilis
5.3.1.24 26300
-
 gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.48 1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate Bacillus subtilis enzyme is involved in tryptophan biosynthesis ?
-
 ?
4.1.1.48 1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate Bacillus subtilis T3 enzyme is involved in tryptophan biosynthesis ?
-
 ?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate Bacillus subtilis involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate Bacillus subtilis T3 involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.48 Bacillus subtilis
-
-
-
5.3.1.24 Bacillus subtilis
-
-
-
5.3.1.24 Bacillus subtilis
-
 not associated with indole-3-glycerol-phosphate synthetase
-
5.3.1.24 Bacillus subtilis T3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.48
-
 Bacillus subtilis
5.3.1.24
-
 Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.48 3.7
-
-
 Bacillus subtilis
5.3.1.24 5.15
-
-
 Bacillus subtilis

Storage Stability

EC Number Storage Stability Organism
4.1.1.48 4°C, in presence of 0.8 M sucrose, 20% loss of activity Bacillus subtilis
5.3.1.24 4°C, 0.8 M sucrose, 7 days, 20% loss of activity, 15 days, 65% loss of activity Bacillus subtilis
5.3.1.24 4°C, 10-15% v/v glycerol, 6-15 days, average of 24% loss of activity Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.48 1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate
-
 Bacillus subtilis 1-(3-Indolyl)glycerol-3-phosphate + CO2 + H2O
-
 ?
4.1.1.48 1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate
-
 Bacillus subtilis T3 1-(3-Indolyl)glycerol-3-phosphate + CO2 + H2O
-
 ?
4.1.1.48 1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate enzyme is involved in tryptophan biosynthesis Bacillus subtilis ?
-
 ?
4.1.1.48 1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate enzyme is involved in tryptophan biosynthesis Bacillus subtilis T3 ?
-
 ?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate
-
 Bacillus subtilis 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate Bacillus subtilis 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate
-
 Bacillus subtilis T3 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate Bacillus subtilis T3 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.48 monomer 1 * 23500, SDS-PAGE Bacillus subtilis
5.3.1.24 monomer 1 * 21800, SDS-PAGE Bacillus subtilis