BRENDA - Enzyme Database

Escherichia coli glyoxalate carboligase

Cromartie, T.H.; Walsh, C.T.; J. Biol. Chem. 251, 329-333 (1976)

Data extracted from this reference:

Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.1.47
68000
-
2 * 68000, SDS-PAGE
Escherichia coli
4.1.1.47
145000
-
gel filtration
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.1.47
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.1.47
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.47
Glyoxylate
-
4584
Escherichia coli
Tartronate semialdehyde + CO2
-
4584
Escherichia coli
-
Subunits
EC Number
Subunits
Commentary
Organism
4.1.1.47
dimer
2 * 68000, SDS-PAGE
Escherichia coli
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
4.1.1.47
1,5-Dihydro-5-deaza-FADH2
reconstitution of the apoenzyme with 50% recovery of the ativity, Km: 0.00071
Escherichia coli
4.1.1.47
5-deaza-FAD
can reconstitute the apoenzyme to full activity, Km: 0.00074 mM
Escherichia coli
4.1.1.47
FAD
1 mol of FAD per mol of dimer, Km: 0.00025 mM; coenzyme
Escherichia coli
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
4.1.1.47
1,5-Dihydro-5-deaza-FADH2
reconstitution of the apoenzyme with 50% recovery of the ativity, Km: 0.00071
Escherichia coli
4.1.1.47
5-deaza-FAD
can reconstitute the apoenzyme to full activity, Km: 0.00074 mM
Escherichia coli
4.1.1.47
FAD
1 mol of FAD per mol of dimer, Km: 0.00025 mM; coenzyme
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.1.47
68000
-
2 * 68000, SDS-PAGE
Escherichia coli
4.1.1.47
145000
-
gel filtration
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.1.47
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.47
Glyoxylate
-
4584
Escherichia coli
Tartronate semialdehyde + CO2
-
4584
Escherichia coli
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.1.47
dimer
2 * 68000, SDS-PAGE
Escherichia coli