Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhou, L.; Rosevear, P.R.
    Mutation of the carboxy terminal zinc finger of E. coli isoleucyl-tRNA synthetase alters zinc binding and aminoacylation activity (1995), Biochem. Biophys. Res. Commun., 216, 648-654.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.5 AIleRS mutant enzymes IleRS(C922S) and AIleRS with replacement of Cys922 through Ala939 with a 33 amino acid peptide unable to bind zinc. Mutant enzymes have altered zinc binding and aminoacylation activity Escherichia coli
6.1.1.5 IleRS(C922S) mutant enzymes IleRS(C922S) and AIleRS with replacement of Cys922 through Ala939 with a 33 amino acid peptide unable to bind zinc. Mutant enzymes have altered zinc binding and aminoacylation activity Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.5 0.0021
-
 tRNAIle wild-type enzyme Escherichia coli
6.1.1.5 0.7
-
 ATP wild-type enzyme Escherichia coli
6.1.1.5 1.3
-
 Ile wild-type enzyme Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.5 Zinc 2 enzyme bound zinc atoms per polypeptide chain Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.5 Escherichia coli
-
 wild-type and mutant enzymes IleRS(C922S) and AIleRS, with replacement of Cys922 through Ala939 with a 33 amino acid peptide unable to bind zinc (AIleRS), mutant enzymes have altered zinc binding and aminoacylation activity
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.5 ATP + L-isoleucine + tRNAIle
-
 Escherichia coli AMP + diphosphate + L-isoleucyl-tRNAIle
-
 ?
6.1.1.5 additional information Ile + ATP + enzyme/Ile-AMP-enzyme + diphosphate, isoleucine-dependent ATP-diphosphate exchange Escherichia coli ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.5 3.1
-
 tRNAIle wild-type enzyme Escherichia coli
6.1.1.5 104
-
 isoleucine wild-type enzyme Escherichia coli