Literature summary extracted from

Bowien, B.; Mayer, F.; Codd, G.A.; Schlegel, H.G.
Purification, some properties and quaternary structure of the D-ribulose 1,5-diphosphate carboxylase of Alcaligens eutrophus (1976), Arch. Microbiol., 110, 157-166.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
4.1.1.39	O2	inhibition of carboxylase	Cupriavidus necator

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4.1.1.39	0.066	-	CO2	-	Cupriavidus necator
4.1.1.39	0.59	-	D-ribulose 1,5-bisphosphate	-	Cupriavidus necator

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
4.1.1.39	cytoplasm	-	Cupriavidus necator	5737	-

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
4.1.1.39	13000	-	L8S8, 8 * 52000 (L) + 8 * 13000 (S), SDS-PAGE, determination of quaternary structure by electron microscopy	Cupriavidus necator
4.1.1.39	52000	-	L8S8, 8 * 52000 (L) + 8 * 13000 (S), SDS-PAGE, determination of quaternary structure by electron microscopy	Cupriavidus necator
4.1.1.39	505000	-	sucrose density gradient centrifugation, gel filtration	Cupriavidus necator

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4.1.1.39	D-Ribulose 1,5-bisphosphate + CO2	Cupriavidus necator	-	?	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.1.1.39	Cupriavidus necator	-	basonym Alcaligenes eutrophus	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
4.1.1.39	-	Cupriavidus necator

Reaction

EC Number	Reaction	Comment	Organism	Reaction ID
4.1.1.39	2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O	D-ribulose 1,5-bisphosphate + CO2 = 2 3-phospho-D-glycerate, carboxylase reaction	Cupriavidus necator	a

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
4.1.1.39	1.56	-	carboxylase	Cupriavidus necator

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4.1.1.39	D-Ribulose 1,5-bisphosphate + CO2	-	Cupriavidus necator	D-3-Phosphoglycerate	-	?
4.1.1.39	D-Ribulose 1,5-bisphosphate + CO2	-	Cupriavidus necator	?	-	?

Subunits

EC Number	Subunits	Comment	Organism
4.1.1.39	hexadecamer	L8S8, 8 * 52000 (L) + 8 * 13000 (S), SDS-PAGE, determination of quaternary structure by electron microscopy	Cupriavidus necator

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Release 2023.1 (January 2023)