Literature summary extracted from

  • Borchardt, R.T.; Cheng, C.F.
    Purification and characterization of rat liver microsomal thiol methyltransferase (1978), Biochim. Biophys. Acta, 522, 340-353.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.9 additional information overview: inhibition by analogs of S-adenosyl-L-homocysteine and analogs of S-adenosyl-L-methionine Rattus norvegicus
2.1.1.9 N-ethylmaleimide more than 50% inhibition at 0.2 mM Rattus norvegicus
2.1.1.9 p-chloromercuribenzoate more than 50% inhibition at 0.1 mM Rattus norvegicus
2.1.1.9 S-3-deazaadenosyl-L-homocysteine
-
Rattus norvegicus
2.1.1.9 S-adenosyl-L-homocysteine
-
Rattus norvegicus
2.1.1.9 S-Tubercidinyl-L-homocysteine
-
Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.9 0.06
-
S-Aristeromycinyl-L-methionine
-
Rattus norvegicus
2.1.1.9 0.12
-
S-3'-Deoxyadenosyl-L-methionine
-
Rattus norvegicus
2.1.1.9 0.154
-
S-Tubercidinyl-L-methionine
-
Rattus norvegicus
2.1.1.9 0.106
-
S-adenosyl-L-methionine
-
Rattus norvegicus
2.1.1.9 0.224
-
S-3-Deazaadenosyl-L-methionine
-
Rattus norvegicus
2.1.1.9 2.37
-
2-mercaptoethanol
-
Rattus norvegicus
2.1.1.9 0.719
-
S-8-Azaadenosyl-L-methionine
-
Rattus norvegicus
2.1.1.9 1.017
-
S-N6-Methyladenosyl-L-methionine
-
Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.9 microsome
-
Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.9 additional information no stimulation by: Na+, K+, Mn2+ Rattus norvegicus
2.1.1.9 additional information no stimulation by: Mg2+ Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.9 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.9 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.9 liver
-
Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
2.1.1.9 4°C, 140 mM NaCl, 10 mM Tris-chloride, pH 7.4, half-life: 4 days, best conditions of storage Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.9 additional information not: glutathione Rattus norvegicus ?
-
?
2.1.1.9 additional information not: cysteine Rattus norvegicus ?
-
?
2.1.1.9 S-adenosyl-L-methionine + a thiol high specificity for S-adenosyl-L-methionine Rattus norvegicus S-adenosyl-L-homocysteine + a thioether
-
?
2.1.1.9 2-mercaptoethanol + S-adenosyl-L-methionine
-
Rattus norvegicus S-methyl-2-mercaptoethanol + S-adenosyl-L-homocysteine
-
?
2.1.1.9 mercaptoacetic acid + S-adenosyl-L-methionine i.e. thioglycolic acid Rattus norvegicus S-methylmercaptoacetic acid + S-adenosyl-L-homocysteine
-
?
2.1.1.9 S-tubercidinyl-L-methionine + 2-mercaptoethanol
-
Rattus norvegicus S-tubercidinyl-L-homocysteine + S-methyl-2-mercaptoethanol
-
?
2.1.1.9 S-3-deazaadenosyl-L-methionine + 2-mercaptoethanol
-
Rattus norvegicus S-3-deazaadenosyl-L-homocysteine + S-methyl-2-mercaptoethanol
-
?
2.1.1.9 S-N6-methyladenosyl-L-methionine + 2-mercaptoethanol
-
Rattus norvegicus S-N6-methyladenosyl-L-homocysteine + S-methyl-2-mercaptoethanol
-
?
2.1.1.9 S-8-azaadenosyl-L-methionine + 2-mercaptoethanol
-
Rattus norvegicus S-8-azaadenosyl-L-homocysteine + S-methyl-2-mercaptoethanol
-
?
2.1.1.9 S-3'-deoxyadenosyl-L-methionine + 2-mercaptoethanol
-
Rattus norvegicus S-3'-deoxyadenosyl-L-homocysteine + S-methyl-2-mercaptoethanol
-
?
2.1.1.9 S-aristeromycinyl-L-methionine + 2-mercaptoethanol
-
Rattus norvegicus S-aristeromycinyl-L-homocysteine + S-methyl-2-mercaptoethanol
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.9 37
-
assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.9 7.8 9 S-methylation of 2-mercaptoethanol Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.1.1.9 7.9
-
30°C, absence of nucleotide substrate, half-life: less than 1 min Rattus norvegicus