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Literature summary extracted from

  • Dhar, K.; Rosazza, J.P.N.
    Purification and characterization of Streptomyces griseus catechol O-methyltransferase (2000), Appl. Environ. Microbiol., 66, 4877-4882.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.6 Hg2+ complete inhibition at 1 mM Streptomyces griseus
2.1.1.6 homocysteine
-
 Streptomyces griseus
2.1.1.6 N-ethylmaleimide complete inhibition at 1 mM Streptomyces griseus
2.1.1.6 p-chloromercuribenzoate complete inhibition at 1 mM Streptomyces griseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.6 additional information
-
 additional information assay method Streptomyces griseus
2.1.1.6 0.5
-
 6,7-dihydroxycoumarin
-
 Streptomyces griseus
2.1.1.6 0.6
-
 S-adenosyl-L-methionine
-
 Streptomyces griseus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.6 Mg2+ required Streptomyces griseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.6 36000
-
 gel filtration Streptomyces griseus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.6 Streptomyces griseus
-
 S-COMT
-
2.1.1.6 Streptomyces griseus S-COMT
-
 S-COMT
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.6
-
 Streptomyces griseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.6 4-nitrocatechol + S-adenosyl-L-methionine
-
 Streptomyces griseus S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.6 4-nitrocatechol + S-adenosyl-L-methionine
-
 Streptomyces griseus S-COMT S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.6 6,7-dihydroxycoumarin + S-adenosyl-L-methionine
-
 Streptomyces griseus S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.6 6,7-dihydroxycoumarin + S-adenosyl-L-methionine
-
 Streptomyces griseus S-COMT S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.6 caffeic acid + S-adenosyl-L-methionine
-
 Streptomyces griseus S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.6 caffeic acid + S-adenosyl-L-methionine
-
 Streptomyces griseus S-COMT S-adenosyl-L-homocysteine + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.6 monomer 1 * 36000, SDS-PAGE, gel filtration Streptomyces griseus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.6 35
-
-
 Streptomyces griseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.6 7.5
-
-
 Streptomyces griseus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.6 0.224
-
 homocysteine
-
 Streptomyces griseus