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Literature summary extracted from

  • Rueffer, M.; Amann, M.; Zenk, M.H.
    S-Adenosyl-L-methionine:columbamine-O-methyl transferase, a compartmentalized enzyme in protoberberine biosynthesis (1986), Plant Cell Rep., 3, 182-185.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.118 S-adenosyl-L-homocysteine
-
 Berberis wilsoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.118 0.0013
-
 Columbamine
-
 Berberis wilsoniae
2.1.1.118 0.0013
-
 Columbamine
-
 Berberis aggregata
2.1.1.118 0.0015
-
 S-adenosyl-L-methionine
-
 Berberis wilsoniae
2.1.1.118 0.0015
-
 S-adenosyl-L-methionine
-
 Berberis aggregata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.118 vesicular fraction
-
 Berberis wilsoniae
-
-
2.1.1.118 vesicular fraction
-
 Berberis aggregata
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.118 52000
-
 gel filtration Berberis wilsoniae
2.1.1.118 52000
-
 gel filtration Berberis aggregata

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.118 Berberis aggregata
-
-
-
2.1.1.118 Berberis wilsoniae
-
 var. subcaulialate
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.118 partial Berberis wilsoniae
2.1.1.118 partial Berberis aggregata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.118 cell suspension culture
-
 Berberis wilsoniae
-
2.1.1.118 cell suspension culture
-
 Berberis aggregata
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.118 S-adenosyl-L-methionine + columbamine
-
 Berberis wilsoniae S-adenosyl-L-homocysteine + palmatine
-
 ?
2.1.1.118 S-adenosyl-L-methionine + columbamine
-
 Berberis aggregata S-adenosyl-L-homocysteine + palmatine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.118 20
-
-
 Berberis wilsoniae
2.1.1.118 20
-
-
 Berberis aggregata