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Literature summary extracted from

  • Akashi, T.; Sawada, Y.; Shimada, N.; Sakurai, N.; Aoki, T.; Ayabe, S.
    cDNA cloning and biochemical characterization of S-adenosyl-L-methionine:2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase, a critical enzyme of the legume isoflavonoid phytoalexin pathway (2003), Plant Cell Physiol., 44, 103-112.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.212 expression in Escherichia coli Glycyrrhiza echinata
2.1.1.212 expression in Escherichia coli Lotus japonicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.212 S-adenosyl-L-methionine + 2,7,4'-trihydroxyisoflavanone Glycyrrhiza echinata the enzyme is involved in formononetin biosynthesis in legumes S-adenosyl-L-homocysteine + 2,7-dihydroxy-4'-methoxyisoflavanone
-
 ?
2.1.1.212 S-adenosyl-L-methionine + 2,7,4'-trihydroxyisoflavanone Lotus japonicus the enzyme is involved in formononetin biosynthesis in legumes S-adenosyl-L-homocysteine + 2,7-dihydroxy-4'-methoxyisoflavanone
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.212 Glycyrrhiza echinata Q84KK6
-
-
2.1.1.212 Lotus japonicus Q84KK4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.212 partial Glycyrrhiza echinata

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.212 49.2
-
 30°C, pH 7.5 Glycyrrhiza echinata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.212 additional information no activity with daidzein Glycyrrhiza echinata ?
-
 ?
2.1.1.212 S-adenosyl-L-methionine + 2,7,4'-trihydroxyisoflavanone
-
 Glycyrrhiza echinata S-adenosyl-L-homocysteine + 2,7-dihydroxy-4'-methoxyisoflavanone
-
 ?
2.1.1.212 S-adenosyl-L-methionine + 2,7,4'-trihydroxyisoflavanone
-
 Lotus japonicus S-adenosyl-L-homocysteine + 2,7-dihydroxy-4'-methoxyisoflavanone
-
 ?
2.1.1.212 S-adenosyl-L-methionine + 2,7,4'-trihydroxyisoflavanone the enzyme is involved in formononetin biosynthesis in legumes Glycyrrhiza echinata S-adenosyl-L-homocysteine + 2,7-dihydroxy-4'-methoxyisoflavanone
-
 ?
2.1.1.212 S-adenosyl-L-methionine + 2,7,4'-trihydroxyisoflavanone the enzyme is involved in formononetin biosynthesis in legumes Lotus japonicus S-adenosyl-L-homocysteine + 2,7-dihydroxy-4'-methoxyisoflavanone
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.212 HI4'OMT
-
 Glycyrrhiza echinata
2.1.1.212 HI4'OMT
-
 Lotus japonicus
2.1.1.212 SAM: 2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase
-
 Lotus japonicus
2.1.1.212 SAM:2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase
-
 Glycyrrhiza echinata