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Literature summary extracted from

  • Liu, C.J.; Dixon, R.A.
    Elicitor-induced association of isoflavone O-methyltransferase with endomembranes prevents the formation and 7-O-methylation of daidzein during isoflavonoid phytoalexin biosynthesis (2001), Plant Cell, 13, 2643-2658.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.150 endoplasmic reticulum IOMT colocalizes with the endoplasmic reticulum–associated isoflavone synthase cytochrome P450 Medicago sativa 5783
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Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.150 S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone Medicago sativa i.e. daidzein. The operationally soluble IOMT localizes to endomembranes after elicitation of the isoflavonoid pathway. IOMT colocalizes with the endoplasmic reticulum–associated isoflavone synthase cytochrome P450 to ensure rapid B-ring methylation of the unstable 2,7,4'-trihydroxyisoflavanone product of isoflavone synthase, thereby preventing its dehydration to daidzein and subsequent A-ring methylation by free IOMT. In this way, metabolic channeling at the entry point into isoflavonoid phytoalexin biosynthesis protects an unstable intermediate from an unproductive metabolic conversion S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone i.e. formononetin ?
2.1.1.150 S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone Medicago sativa cv Regen SY i.e. daidzein. The operationally soluble IOMT localizes to endomembranes after elicitation of the isoflavonoid pathway. IOMT colocalizes with the endoplasmic reticulum–associated isoflavone synthase cytochrome P450 to ensure rapid B-ring methylation of the unstable 2,7,4'-trihydroxyisoflavanone product of isoflavone synthase, thereby preventing its dehydration to daidzein and subsequent A-ring methylation by free IOMT. In this way, metabolic channeling at the entry point into isoflavonoid phytoalexin biosynthesis protects an unstable intermediate from an unproductive metabolic conversion S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone i.e. formononetin ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.150 Medicago sativa O24529
-
-
2.1.1.150 Medicago sativa cv Regen SY O24529
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.150 cell suspension culture
-
 Medicago sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.150 S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone i.e. daidzein. The operationally soluble IOMT localizes to endomembranes after elicitation of the isoflavonoid pathway. IOMT colocalizes with the endoplasmic reticulum–associated isoflavone synthase cytochrome P450 to ensure rapid B-ring methylation of the unstable 2,7,4'-trihydroxyisoflavanone product of isoflavone synthase, thereby preventing its dehydration to daidzein and subsequent A-ring methylation by free IOMT. In this way, metabolic channeling at the entry point into isoflavonoid phytoalexin biosynthesis protects an unstable intermediate from an unproductive metabolic conversion Medicago sativa S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone i.e. formononetin ?
2.1.1.150 S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone i.e. daidzein. The activity of alfalfa IOMT as an elicitor-inducible, operationally soluble enzyme with 7-position specificity for daidzein in vitro Medicago sativa S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone i.e. formononetin ?
2.1.1.150 S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone i.e. daidzein. The operationally soluble IOMT localizes to endomembranes after elicitation of the isoflavonoid pathway. IOMT colocalizes with the endoplasmic reticulum–associated isoflavone synthase cytochrome P450 to ensure rapid B-ring methylation of the unstable 2,7,4'-trihydroxyisoflavanone product of isoflavone synthase, thereby preventing its dehydration to daidzein and subsequent A-ring methylation by free IOMT. In this way, metabolic channeling at the entry point into isoflavonoid phytoalexin biosynthesis protects an unstable intermediate from an unproductive metabolic conversion Medicago sativa cv Regen SY S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone i.e. formononetin ?
2.1.1.150 S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone i.e. daidzein. The activity of alfalfa IOMT as an elicitor-inducible, operationally soluble enzyme with 7-position specificity for daidzein in vitro Medicago sativa cv Regen SY S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone i.e. formononetin ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.150 IOMT8
-
 Medicago sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.150 30
-
 assay at Medicago sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.150 7.5
-
 assay at Medicago sativa