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Literature summary extracted from

  • He, X.Z.; Reddy, J.T.; Dixon, R.A.
    Stress responses in alfalfa (Medicago sativa L). XXII. cDNA cloning and characterization of an elicitor-inducible isoflavone 7-O-methyltransferase (1998), Plant Mol. Biol., 36, 43-54.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.150 expression in Escherichia coli Medicago sativa
2.1.1.270 expression in Escherichia coli Medicago sativa

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.150 up IOMT transcripts are rapidly induced following elicitation, prior to the increase in IOMT activity Medicago sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.150 39603
-
 x * 39603, calculated from sequence Medicago sativa
2.1.1.150 41000
-
 x * 41000, SDS-PAGE Medicago sativa
2.1.1.270 41000
-
 x * 41000, SDS-PAGE, recombinant enzyme Medicago sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.150 S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone Medicago sativa i.e. daidzein S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone i.e. isoformononetin ?
2.1.1.270 additional information Medicago sativa in vivo reaction is 4'-O-methylation of isoflavones, in vitro reaction is 7-O-methylation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.150 Medicago sativa O24529
-
-
2.1.1.270 Medicago sativa O24529
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.270
-
 Medicago sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.150 callus
-
 Medicago sativa
-
2.1.1.150 cell suspension culture
-
 Medicago sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.150 S-adenosyl-L-methionine + 5,7,4'-trihydroxyisoflavone i.e. genistein. 12.9% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein) Medicago sativa S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyisoflavone
-
 ?
2.1.1.150 S-adenosyl-L-methionine + 6,7,4'-trihydroxyisoflavone 136% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein) Medicago sativa S-adenosyl-L-homocysteine + 6,4'-dihydroxy-7-methoxyisoflavone
-
 ?
2.1.1.150 S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone i.e. daidzein Medicago sativa S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone i.e. isoformononetin ?
2.1.1.270 additional information in vivo reaction is 4'-O-methylation of isoflavones, in vitro reaction is 7-O-methylation Medicago sativa ?
-
 ?
2.1.1.270 S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain at 49% of the activity with daidzein, the enzyme also catalyzes the reaction of EC 2.1.1.150 Medicago sativa S-adenosyl-L-homocysteine + (+)-pisatin
-
 ?
2.1.1.270 S-adenosyl-L-methionine + (+)-maackiain at 1.7% of the activity with daidzein, the enzyme also catalyzes the reaction of EC 2.1.1.150 Medicago sativa S-adenosyl-L-homocysteine + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.150 ? x * 41000, SDS-PAGE Medicago sativa
2.1.1.150 ? x * 39603, calculated from sequence Medicago sativa
2.1.1.270 ? x * 41000, SDS-PAGE, recombinant enzyme Medicago sativa

Synonyms

EC Number Synonyms Comment Organism
2.1.1.150 7-IOMT
-
 Medicago sativa
2.1.1.150 IOMT8
-
 Medicago sativa
2.1.1.270 IOMT8
-
 Medicago sativa

Expression

EC Number Organism Comment Expression
2.1.1.270 Medicago sativa IOMT8 transcripts are rapidly induced following elicitation up