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Literature summary extracted from

  • He, X.Z.; Dixon, R.A.
    Affinity chromatography, substrate/product specificity, and amino acid sequence analysis of an isoflavone O-methyltransferase from alfalfa (Medicago sativa L.) (1996), Arch. Biochem. Biophys., 336, 121-129.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.150 S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone Medicago sativa i.e. daidzein, about 20% of the activity with 6,7,4'-trihydroxyisoflavone (i.e. glycitein). Key enzyme in the biosynthesis of the phytoalexin medicarpin in Medicago sativa S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone i.e. formononetin ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.150 Medicago sativa O24529
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.150
-
 Medicago sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.150 callus
-
 Medicago sativa
-
2.1.1.150 cell suspension culture
-
 Medicago sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.150 50
-
 pH 8.5, 30°C Medicago sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.150 additional information no activity with apigenin (a flavone), naringenine (a falvanone) Medicago sativa ?
-
 ?
2.1.1.150 S-adenosyl-L-methionine + 5,7,4'-trihydroxyisoflavone i.e. genistein, about 7% of the activity with 6,7,4'-trihydroxyisoflavone (i.e. glycitein) Medicago sativa S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyisoflavone
-
 ?
2.1.1.150 S-adenosyl-L-methionine + 6,7,4'-trihydroxyisoflavone (i.e. glycitein) Medicago sativa S-adenosyl-L-homocysteine + 6,4'-dihydroxy-7-methoxyisoflavone
-
 ?
2.1.1.150 S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone i.e. daidzein, about 20% of the activity with 6,7,4'-trihydroxyisoflavone (i.e. glycitein). Key enzyme in the biosynthesis of the phytoalexin medicarpin in Medicago sativa Medicago sativa S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone i.e. formononetin ?
2.1.1.150 S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone i.e. daidzein, about 20% of the activity with 6,7,4'-trihydroxyisoflavone (i.e. glycitein) Medicago sativa S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone i.e. formononetin ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.150 8-IOMT
-
 Medicago sativa
2.1.1.150 isoflavone O-methyltransferase
-
 Medicago sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.150 30
-
 assay at Medicago sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.150 8.5
-
 glycine-NaOH buffer Medicago sativa