EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.2.1 | expression in Escherichia coli | Geobacillus stearothermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.2.1 | 0.7 | - |
L-serine | only tetrameric form | Geobacillus stearothermophilus | |
2.1.2.1 | 0.9 | - |
L-serine | only dimeric form | Geobacillus stearothermophilus | |
2.1.2.1 | 1 | - |
L-serine | - |
Geobacillus stearothermophilus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.2.1 | 90000 | - |
gel filtration | Geobacillus stearothermophilus |
2.1.2.1 | 90000 | - |
dimeric form | Geobacillus stearothermophilus |
2.1.2.1 | 180000 | - |
tetrameric form, gel filtration | Geobacillus stearothermophilus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.1 | L-serine + tetrahydrofolate | Geobacillus stearothermophilus | - |
glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.2.1 | Geobacillus stearothermophilus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.2.1 | gene cloned and expressed in Escherichia coli | Geobacillus stearothermophilus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.1.2.1 | 5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine | mechanism | Geobacillus stearothermophilus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.2.1 | 4.1 | - |
only tetrameric form | Geobacillus stearothermophilus |
2.1.2.1 | 4.2 | - |
- |
Geobacillus stearothermophilus |
2.1.2.1 | 6.7 | - |
only dimeric form | Geobacillus stearothermophilus |
2.1.2.1 | 32.4 | - |
dimeric form at 80°C | Geobacillus stearothermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.1 | L-serine + tetrahydrofolate | - |
Geobacillus stearothermophilus | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
2.1.2.1 | additional information | enzyme catalyses the racemization of D- and L-alanine | Geobacillus stearothermophilus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.2.1 | More | 90% dimer and 10% tetramer in the same organism, one subunit: 45000 Da, SDS-PAGE | Geobacillus stearothermophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.2.1 | 80 | - |
dimeric form of enzyme | Geobacillus stearothermophilus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.2.1 | additional information | - |
Tm values of enzyme and dimeric and tetrameric forms in the absence and presence of ligands | Geobacillus stearothermophilus |
2.1.2.1 | additional information | - |
pyridoxal 5'-phosphate increases the thermal stability | Geobacillus stearothermophilus |
2.1.2.1 | additional information | - |
L-serine increases the thermal stability | Geobacillus stearothermophilus |
2.1.2.1 | 60 | - |
enzyme stable in the absence of any ligand | Geobacillus stearothermophilus |
2.1.2.1 | 70 | - |
50% loss of activity in the absence of any ligand | Geobacillus stearothermophilus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.2.1 | 3.6 | - |
L-serine | only tetrameric form | Geobacillus stearothermophilus | |
2.1.2.1 | 4.3 | - |
L-serine | - |
Geobacillus stearothermophilus | |
2.1.2.1 | 5 | - |
L-serine | only dimeric form | Geobacillus stearothermophilus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.2.1 | pyridoxal 5'-phosphate | - |
Geobacillus stearothermophilus |