Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rao, J.V.K.; Prakash, V.; Appaji Rao, N.; Savithri, H.S.
    The role of Glu74 and Tyr82 in the reaction catalyzed by sheep liver cytosolic serine hydroxymethyltransferase (2000), Eur. J. Biochem., 267, 5967-5976.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.2.1 medicine enzyme is a potential target for cancer chemotherapy Ovis aries

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.1 expression in Escherichia coli Ovis aries

Protein Variants

EC Number Protein Variants Comment Organism
2.1.2.1 E74K specific activities drastically reduced with serine as substrate, but D-alanine transamination and allothreonine cleavage at rates comparable with wild-type enzyme Ovis aries
2.1.2.1 E74Q specific activities drastically reduced with serine as substrate, but D-alanine transamination and allothreonine cleavage at rates comparable with wild type enzyme Ovis aries
2.1.2.1 Y82F 95% loss of activity, kcat and Km decreased, a role in stabilizing the quinonoid intermediate Ovis aries

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.1 0.15 0.9 L-serine mutant enzyme Y82F Ovis aries
2.1.2.1 1
-
 L-serine
-
 Ovis aries
2.1.2.1 1.5
-
 L-serine mutant enzyme E74Q Ovis aries

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.1 53000
-
 4 * 53000, mutant enzyme Ovis aries
2.1.2.1 220000
-
 mutant enzyme Ovis aries

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.1 L-serine + tetrahydrofolate Ovis aries
-
 glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Ovis aries
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine mechanism Ovis aries
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.1 0.012
-
 mutant enzyme E74Q Ovis aries
2.1.2.1 0.16
-
 mutant enzyme Y82F Ovis aries
2.1.2.1 4.2
-
-
 Ovis aries

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 L-serine + tetrahydrofolate
-
 Ovis aries glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?
2.1.2.1 additional information enzyme catalyzes the pyridoxal 5'-phosphate dependent reversible cleavage of 3-hydroxy-alpha-amino acids Ovis aries ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.2.1 dimer removal of the bound pyridoxal 5'-phosphate from the mutant tetrameric enzyme leads to dissociation to a dimer Ovis aries
2.1.2.1 homotetramer 4 * 53000, mutant enzyme Ovis aries

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.1.2.1 additional information
-
 thermograms of enzyme and mutant enzyme in the absence and presence of L-serine Ovis aries

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.2.1 additional information
-
 Tm: 55°C, increased to 65°C in the presence of L-serine, Tm: 68°C mutant enzyme Y82F in the presence of serine Ovis aries
2.1.2.1 additional information
-
 L-serine increases the thermal stability Ovis aries

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.2.1 0.01
-
 L-serine mutant enzyme E74Q Ovis aries
2.1.2.1 0.0967
-
 L-serine mutant enzyme Y82F Ovis aries
2.1.2.1 4.1
-
 L-serine
-
 Ovis aries

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 pyridoxal 5'-phosphate
-
 Ovis aries