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Literature summary extracted from

  • Schirch, V.; Hopkins, S.; Villar, E.; Angelaccio, S.
    Serine hydroxymethyltransferase from Escherichia coli: purification and properties (1985), J. Bacteriol., 163, 1-7.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.1 Escherichia coli glyA gene Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.1 5,5-dithiobis(2-nitrobenzoic acid)
-
 Escherichia coli
2.1.2.1 D-alanine inactivates enzyme by converting the enzyme bound pyridoxal 5'-phosphate to pyridoxamine phosphate in a transamination reaction Escherichia coli
2.1.2.1 methyl methanethiosulfonate
-
 Escherichia coli
2.1.2.1 additional information not: iodoacetate Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.1 additional information
-
 additional information
-
 Escherichia coli
2.1.2.1 0.08
-
 tetrahydrofolate
-
 Escherichia coli
2.1.2.1 0.15 0.9 L-serine
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.1 46000
-
 2 * 46000, SDS-PAGE Escherichia coli
2.1.2.1 47000
-
 2 * 47000, calculated from DNA-sequence Escherichia coli
2.1.2.1 96000
-
 sedimentation equilibrium centrifugation, amino acid composition Escherichia coli
2.1.2.1 100000 115000 HPLC gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.1 L-serine + tetrahydrofolate Escherichia coli major pathway for production of C1-units of 5,10-methylenetetrahydrofolate glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.1
-
 Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine mechanism Escherichia coli
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.1 13.6
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 L-serine + tetrahydrofolate
-
 Escherichia coli glycine + 5,10-methylenetetrahydrofolate + H2O
-
 r
2.1.2.1 L-serine + tetrahydrofolate major pathway for production of C1-units of 5,10-methylenetetrahydrofolate Escherichia coli glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?
2.1.2.1 additional information enzyme transaminates D-alanine to pyruvate and pyridoxamine phosphate Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.2.1 dimer 2 * 46000, SDS-PAGE Escherichia coli
2.1.2.1 dimer 2 * 47000, calculated from DNA-sequence Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.2.1 additional information
-
 additional information
-
 Escherichia coli
2.1.2.1 10.7
-
 L-serine
-
 Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.1.2.1 6 8.5 stable Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 pyridoxal 5'-phosphate
-
 Escherichia coli
2.1.2.1 tetrahydrofolate requirement with L-serine or L-2-methylserine as substrate Escherichia coli
2.1.2.1 tetrahydrofolate derivatives requirement, if L-serine is substrate Escherichia coli