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Literature summary extracted from

  • Mitchell, M.K.; Reynolds, P.H.S.; Blevins, D.G.
    Serine hydroxymethyltransferase from soybean root nodules (1986), Plant Physiol., 81, 553-557.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.1.2.1 L-serine stabilizes Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.1 5,10-methenyltetrahydrofolate
-
 Glycine max
2.1.2.1 5,10-methylene-5,6,7,8-tetrahydrofolic acid
-
 Glycine max
2.1.2.1 5-methyl-5,6,7,8-tetrahydrofolate
-
 Glycine max
2.1.2.1 glycine
-
 Glycine max
2.1.2.1 KCl
-
 Glycine max
2.1.2.1 additional information not: methionine, S-adenosyl-L-methionine, XMP, IMP, phosphoglycerate Glycine max
2.1.2.1 NaCl
-
 Glycine max
2.1.2.1 tetrahydrofolate derivatives
-
 Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.1 additional information
-
 additional information
-
 Glycine max
2.1.2.1 0.25
-
 tetrahydrofolate
-
 Glycine max
2.1.2.1 1.5
-
 L-serine
-
 Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.1 55000
-
 4 * 55000, SDS-PAGE Glycine max
2.1.2.1 230000
-
-
 Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.1 L-serine + tetrahydrofolate Glycine max main glycine source for purine biosynthetic pathway in ureide biogenesis glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?
2.1.2.1 L-serine + tetrahydrofolate Glycine max L. Merr. cv. Williams main glycine source for purine biosynthetic pathway in ureide biogenesis glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Glycine max
-
 soybean
-
2.1.2.1 Glycine max L. Merr. cv. Williams
-
 soybean
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.1
-
 Glycine max

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine mechanism Glycine max
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.2.1 nodule inoculated with Rhizobium japonicum strain 311b142 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.1 additional information
-
-
 Glycine max
2.1.2.1 11.9
-
-
 Glycine max

Storage Stability

EC Number Storage Stability Organism
2.1.2.1 -20°C, 8 weeks Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 L-serine + tetrahydrofolate
-
 Glycine max glycine + 5,10-methylenetetrahydrofolate + H2O
-
 r
2.1.2.1 L-serine + tetrahydrofolate main glycine source for purine biosynthetic pathway in ureide biogenesis Glycine max glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?
2.1.2.1 L-serine + tetrahydrofolate
-
 Glycine max L. Merr. cv. Williams glycine + 5,10-methylenetetrahydrofolate + H2O
-
 r
2.1.2.1 L-serine + tetrahydrofolate main glycine source for purine biosynthetic pathway in ureide biogenesis Glycine max L. Merr. cv. Williams glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.2.1 homotetramer 4 * 55000, SDS-PAGE Glycine max

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.2.1 additional information
-
 additional information
-
 Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.2.1 8.5
-
-
 Glycine max

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.2.1 7.3 9.5 about half-maximal activity at pH 7.3 and about 60% of maximal activity at 9.5 Glycine max
2.1.2.1 9.5
-
 no activity above Glycine max

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.2.1 1.8
-
 5-methyl-5,6,7,8-tetrahydrofolate
-
 Glycine max
2.1.2.1 2.9
-
 5,10-methylene-5,6,7,8-tetrahydrofolic acid
-
 Glycine max
2.1.2.1 3
-
 glycine
-
 Glycine max
2.1.2.1 3
-
 5,10-methenyltetrahydrofolate
-
 Glycine max