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Literature summary extracted from

  • Ranocha, P.; Bourgis, F.; Ziemak, M.J.; Rhodes, D.; Gage, D.A.; Hanson, A.D.
    Characterization and functional expression of cDNAs encoding methionine-sensitive and -insensitive homocysteine S-methyltransferases from Arabidopsis (2000), J. Biol. Chem., 275, 15962-15968.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.10 expression in Escherichia coli Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.10 EDTA 1 mM, 26% inhibition Arabidopsis thaliana
2.1.1.10 L-methionine strong product inhibition Arabidopsis thaliana
2.1.1.10 additional information no product inhibition by L-methionine below 0.5 mM, no inhibition by EDTA Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.10 0.018
-
 L-homocysteine pH 7.5, 30°C, cosubstrate: S-adenosyl-L-methionine Arabidopsis thaliana
2.1.1.10 0.029
-
 S-methyl-L-methionine pH 7.5, 30°C Arabidopsis thaliana
2.1.1.10 0.035
-
 L-homocysteine pH 7.5, 30°C, cosubstrate: S-methyl-L-methionine Arabidopsis thaliana
2.1.1.10 0.05
-
 S-methyl-L-methionine pH 7.5, 30°C Arabidopsis thaliana
2.1.1.10 0.067
-
 L-homocysteine pH 7.5, 30°C, cosubstrate: S-adenosyl-L-methionine Arabidopsis thaliana
2.1.1.10 0.077
-
 L-homocysteine pH 7.5, 30°C, cosubstrate: S-methyl-L-methionine Arabidopsis thaliana
2.1.1.10 0.225
-
 S-adenosyl-L-methionine pH 7.5, 30°C Arabidopsis thaliana
2.1.1.10 1.95
-
 S-adenosyl-L-methionine pH 7.5, 30°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.10 cytosol
-
 Arabidopsis thaliana 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.10 additional information contains a zinc-binding motif, no stimulation by 0.1 or 1 mM Zn2+ Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.10 36000
-
 gel filtration Arabidopsis thaliana
2.1.1.10 36400
-
 1 * 36400, calculated from sequence Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.10 S-adenosyl-L-methionine + L-homocysteine Arabidopsis thaliana the enzyme uses S-methyl-L-methionine and S-adenosyl-L-methionine as methyl donor in vivo S-adenosyl-L-homocysteine + L-methionine
-
 ?
2.1.1.10 S-methyl-L-methionine + L-homocysteine Arabidopsis thaliana the enzyme uses S-methyl-L-methionine and S-adenosyl-L-methionine as methyl donor in vivo 2 L-methionine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.10 Arabidopsis thaliana Q9M1W4
-
-
2.1.1.10 Arabidopsis thaliana Q9SDL7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.10 additional information no activity with D-cysteine, L-cysteine, DL-selenocysteine, glutathione, coenzyme A, sulfide, or thiocyanate Arabidopsis thaliana ?
-
 ?
2.1.1.10 additional information no activity with DL-selenocysteine, glutathione, coenzyme A, sulfide, or thiocyanate Arabidopsis thaliana ?
-
 ?
2.1.1.10 S-adenosyl-L-methionine + L-homocysteine the enzyme uses S-methyl-L-methionine and S-adenosyl-L-methionine as methyl donor in vivo Arabidopsis thaliana S-adenosyl-L-homocysteine + L-methionine
-
 ?
2.1.1.10 S-adenosyl-L-methionine + L-homocysteine the Km-value for S-adenosyl-L-methionine is higher than for S-methyl-L-methionine and the Vmax value is lower Arabidopsis thaliana S-adenosyl-L-homocysteine + L-methionine
-
 ?
2.1.1.10 S-methyl-L-methionine + L-cysteine
-
 Arabidopsis thaliana ?
-
 ?
2.1.1.10 S-methyl-L-methionine + L-homocysteine
-
 Arabidopsis thaliana 2 L-methionine
-
 ?
2.1.1.10 S-methyl-L-methionine + L-homocysteine the enzyme uses S-methyl-L-methionine and S-adenosyl-L-methionine as methyl donor in vivo Arabidopsis thaliana 2 L-methionine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.10 monomer 1 * 36000, calculated from sequence Arabidopsis thaliana
2.1.1.10 monomer 1 * 36400, calculated from sequence Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.1.1.10 AtHMT-1
-
 Arabidopsis thaliana
2.1.1.10 AtHMT-1 encoded by single copy gene Arabidopsis thaliana
2.1.1.10 AtHMT-2
-
 Arabidopsis thaliana
2.1.1.10 AtHMT-2 encoded by single copy gene Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.10 7.5
-
-
 Arabidopsis thaliana