Literature summary extracted from
Nordlund, M.E.; Johansson, J.O.M.; von Pawel-Rammingen, U.; Byström, A.S.
Identification of the TRM2 gene encoding the tRNA(m5U54)methyltransferase of Saccharomyces cerevisiae (2000), RNA, 6, 844-860.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.1.1.35 |
cloning and sequencing of the TRM2 gene encoding enzyme, gene is previously identified as RNC1/NUD1 gene, but does not encode the yNucR endo-exonuclease, TRM2 gene is not essential for cell viability and contains alternative translational start sites, glutathione S-transferase tagged recombinant, Trm2p, is expressed in Escherichia coli |
Saccharomyces cerevisiae |
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
2.1.1.35 |
nucleus |
the m5U modification is found in unspliced tRNAs, suggesting that enzyme action is within the nucleus |
Saccharomyces cerevisiae |
5634 |
- |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
2.1.1.35 |
S-adenosyl-L-methionine + uridine54 in tRNA |
Saccharomyces cerevisiae |
- |
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA |
enzyme is responsible for the formation of 5-methyluridine at position 54 in tRNA |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.1.1.35 |
Saccharomyces cerevisiae |
- |
several strains |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.1.1.35 |
purification of glutathione S-transferase tagged recombinant, Trm2p, expressed in Escherichia coli BL21 |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.1.1.35 |
S-adenosyl-L-methionine + uridine54 in tRNA |
- |
Saccharomyces cerevisiae |
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA |
enzyme is responsible for the formation of 5-methyluridine at position 54 in tRNA |
? |
|
2.1.1.35 |
S-adenosyl-L-methionine + uridine54 in tRNA |
methyl group acceptor: yeast tRNA |
Saccharomyces cerevisiae |
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA |
nearly all tRNAs contain m5U54, initiators excluded |
? |
|
2.1.1.35 |
S-adenosyl-L-methionine + uridine54 in tRNA |
methyl group acceptor: m5U-deficient tRNA from E. coli GB1-5-39 |
Saccharomyces cerevisiae |
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA |
nearly all tRNAs contain m5U54, initiators excluded |
? |
|
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
2.1.1.35 |
37 |
- |
assay at |
Saccharomyces cerevisiae |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
2.1.1.35 |
S-adenosyl-L-methionine |
- |
Saccharomyces cerevisiae |
|