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Literature summary extracted from

  • Gu, X.; Ofengand, J.; Santi, D.V.
    In vitro methylation of Escherichia coli 16S rRNA by tRNA (m5U54)-methyltransferase (1994), Biochemistry, 33, 2255-2261.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.35 trmA gene encodes enzyme, gene is essential for the organism Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.35 5-Fluorouracil substituted tRNA potent inhibitor Escherichia coli
2.1.1.35 tRNAPhe inhibits binding of 16S rRNA to RUMT Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.35 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.35 additional information enzyme also catalyzes exchange of tritium of [3H]-Ura-16S rRNA and protons of water Escherichia coli additional information
-
 ?
2.1.1.35 S-adenosyl-L-methionine + 16S rRNA methyl group acceptor: E. coli 16S rRNA, site of methylation is U788 which is within a 9-base loop of a predicted stem-loop structure, mechanism is proposed to involve formation of a covalent, reversible Michael adduct with the target U788, poor substrate: methylation of 16S rRNA 40fold slower than of tRNAPhe Escherichia coli S-adenosyl-L-homocysteine + 16S rRNA containing thymine in vitro product m5U is not normally found in wild-type 16S rRNA, U788 is not highly methylated in vivo, 1 mol m5U per mol of 16S rRNA ?
2.1.1.35 S-adenosyl-L-methionine + uridine54 in tRNA substrate consensus sequence for the T-arm of tRNA consists of a 2-5 base-pair stem and a 7-base loop Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
 ?
2.1.1.35 S-adenosyl-L-methionine + uridine54 in tRNA methylates U54 in the TPsiC-loop of tRNA Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.35 37
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.35 S-adenosyl-L-methionine
-
 Escherichia coli