Literature summary extracted from
Kealey, J.T.; Santi, D.V.
Stereochemistry of tRNA(m5U54)-methyltransferase catalysis: 19F NMR spectroscopy of an enzyme-FUraRNA covalent complex (1995), Biochemistry, 34, 2441-2446.
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.1.1.35 |
5-Fluorouracil substituted tRNA |
forms inhibitory stable methylated covalent complex with RUMT |
Escherichia coli |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.1.1.35 |
Escherichia coli |
- |
- |
- |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
2.1.1.35 |
S-adenosyl-L-methionine + uracil54 in tRNA = S-adenosyl-L-homocysteine + 5-methyluracil54 in tRNA |
catalytic mechanism |
Escherichia coli |
|
2.1.1.35 |
S-adenosyl-L-methionine + uracil54 in tRNA = S-adenosyl-L-homocysteine + 5-methyluracil54 in tRNA |
steric course of methyl transfer |
Escherichia coli |
|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.1.1.35 |
S-adenosyl-L-methionine + uridine54 in tRNA |
methyl group acceptors: small RNA oligomers corresponding to the T-arm of tRNA, 17-oligomer |
Escherichia coli |
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA |
formation of 5-methyluridine in position 54 of tRNA, in the TPsiC sequence in loop IV |
? |
|
2.1.1.35 |
S-adenosyl-L-methionine + uridine54 in tRNA |
methyl group acceptor: tRNA from E. coli |
Escherichia coli |
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA |
formation of 5-methyluridine in position 54 of tRNA, in the TPsiC sequence in loop IV |
? |
|
2.1.1.35 |
S-adenosyl-L-methionine + uridine54 in tRNA |
methylates U54 in the TPsiC-loop of tRNA |
Escherichia coli |
S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA |
formation of 5-methyluridine in position 54 of tRNA, in the TPsiC sequence in loop IV |
? |
|
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
2.1.1.35 |
S-adenosyl-L-methionine |
- |
Escherichia coli |
|