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Literature summary extracted from

  • Gu, X.; Matsuda, A.; Ivanetich, K.M.; Santi, D.V.
    Interaction of tRNA (uracil-5-)-methyltransferase with NO2Ura-tRNA (1996), Nucleic Acids Res., 24, 1059-1064.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.35 5-Fluorouracil substituted tRNA
-
 Escherichia coli
2.1.1.35 NO2Ura-tRNA potent mechanism-based inhibitor, competitive with respect to tRNA, can be reversed by dilution into a large excess of tRNA substrate, forms a reversible covalent complex in which Cys-324 of RUMT is linked to the 6-position of NO2-Ura 54 in NO2-Ura-tRNA, mechanism Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.35 0.00037
-
 uracil54 in yeast tRNA-Phe
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.35 Escherichia coli
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.35 S-adenosyl-L-methionine + uracil54 in tRNA = S-adenosyl-L-homocysteine + 5-methyluracil54 in tRNA catalytic mechanism Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.35 S-adenosyl-L-methionine + uracil54 in yeast tRNA-Phe
-
 Escherichia coli S-adenosyl-L-homocysteine + 5-methyluracil54 in yeast tRNA-Phe
-
 ?
2.1.1.35 S-adenosyl-L-methionine + uracil54 in yeast tRNA1-Phe
-
 Escherichia coli S-adenosyl-L-homocysteine + 5-methyluracil54 in yeast tRNA1-Phe
-
 r
2.1.1.35 S-adenosyl-L-methionine + uridine54 in tRNA methyl group acceptor: unmodified yeast tRNAPhe Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.35 15
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.35 0.0317
-
 uracil54 in yeast tRNA1-Phe
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.35 S-adenosyl-L-methionine
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.35 0.001
-
 NO2Ura-tRNA
-
 Escherichia coli