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Literature summary extracted from

  • Tscherne, J.S.; Wainfan, E.
    Selective inhibition of uracil tRNA methylases of E. coli by ethionine (1978), Nucleic Acids Res., 5, 451-461.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.35 ethylthioadenosine 0.2 mM Escherichia coli
2.1.1.35 L-ethionine selective inhibitor of uracil tRNA methylase, non-competitive to S-adenosyl-L-methionine Escherichia coli
2.1.1.35 L-methionine 30 mM, weak Escherichia coli
2.1.1.35 additional information not inhibited by alanine, valine, ethionine sulfoxide Escherichia coli
2.1.1.35 norleucine 30 mM, weak Escherichia coli
2.1.1.35 S-adenosyl-L-ethionine 0.6 mM Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.35 S-adenosyl-L-methionine + uridine54 in tRNA Escherichia coli specific inhibition of enzyme by ethionine in vivo S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.35 Escherichia coli
-
 strains B and M3S
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.35 S-adenosyl-L-methionine + uridine54 in tRNA methyl group acceptor: thymine-deficient tRNA from E. coli trmA-mutant Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
 ?
2.1.1.35 S-adenosyl-L-methionine + uridine54 in tRNA methyl group acceptor: thymine-deficient tRNA from E. coli Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
 ?
2.1.1.35 S-adenosyl-L-methionine + uridine54 in tRNA specific inhibition of enzyme by ethionine in vivo Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.35 35
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.35 S-adenosyl-L-methionine
-
 Escherichia coli