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Literature summary extracted from

  • Gu, X.; Santi, D.V.
    The T-arm of tRNA is a substrate for tRNA (m5U54)-methyltransferase (1991), Biochemistry, 30, 2999-3002.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.35 0.0008
-
uracil54 in yeast tRNA1-Phe
-
Escherichia coli
2.1.1.35 0.004
-
uracil54 in 11mer of the T-arm of E. coli tRNA1-Val
-
Escherichia coli
2.1.1.35 0.005
-
uracil54 in 17mer of the T-arm of E. coli tRNA1-Val
-
Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.35 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.35 S-adenosyl-L-methionine + uracil54 in 11mer of the T-arm of E. coli tRNA1-Val
-
Escherichia coli S-adenosyl-L-homocysteine + 5-methyluracil54 in 11mer of the T-arm of E. coli tRNA1-Val
-
?
2.1.1.35 S-adenosyl-L-methionine + uracil54 in 17mer of the T-arm of E. coli tRNA1-Val
-
Escherichia coli S-adenosyl-L-homocysteine + 5-methyluracil54 in 17mer of the T-arm of E. coli tRNA1-Val
-
?
2.1.1.35 S-adenosyl-L-methionine + uracil54 in yeast tRNA1-Phe
-
Escherichia coli S-adenosyl-L-homocysteine + 5-methyluracil54 in yeast tRNA1-Phe
-
?
2.1.1.35 S-adenosyl-L-methionine + uridine54 in tRNA methyl group acceptors: E. coli tRNA1Val, 17-oligomer and 11-oligomer of the T-arm of tRNA1Val, essential sequence for tRNA binding: within the T-arm, composed of 7-base T-loop and 5-base pair stem, includes position 52 of T-stem, the T-loop and extends to the 3'-acceptor end, methylation site at U54 Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
?
2.1.1.35 S-adenosyl-L-methionine + uridine54 in tRNA methyl group acceptor: unmodified yeast tRNAPhe Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
?
2.1.1.35 S-adenosyl-L-methionine + uridine54 in tRNA methyl group acceptors: E. coli tRNAPhe, tRNAVal Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.35 15
-
assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.35 0.0015
-
uracil54 in 11mer of the T-arm of E. coli tRNA1-Val
-
Escherichia coli
2.1.1.35 0.0333
-
uracil54 in 17mer of the T-arm of E. coli tRNA1-Val
-
Escherichia coli
2.1.1.35 0.0917
-
uracil54 in yeast tRNA1-Phe
-
Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.35 S-adenosyl-L-methionine
-
Escherichia coli