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Literature summary extracted from

  • Greenberg, R.; Dudock, B.
    Isolation and characterization of m5U-methyltransferase from Escherichia coli (1980), J. Biol. Chem., 255, 8296-8302.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.35 spermidine slight activation at 20 mM in presence of 50 mM ammonium acetate Escherichia coli

General Stability

EC Number General Stability Organism
2.1.1.35 freeze-thawing inactivates Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.35 S-adenosyl-L-homocysteine at 0.002 mM 50% inhibition, E. coli MRE 600 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.35 0.0011
-
 uracil54 in wheat germ tRNA1-Gly
-
 Escherichia coli
2.1.1.35 0.0125
-
 S-adenosyl-L-methionine
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.35 soluble
-
 Escherichia coli
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.35 ammonium acetate 30% activation at 50 mM Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.35 38000
-
 gel filtration Escherichia coli
2.1.1.35 42000
-
 1 * 42000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.35 S-adenosyl-L-methionine + uridine54 in tRNA Escherichia coli tRNA modifying enzyme S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
 ?
2.1.1.35 S-adenosyl-L-methionine + uridine54 in tRNA Escherichia coli MRE 600 tRNA modifying enzyme S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
 ?
2.1.1.35 S-adenosyl-L-methionine + uridine54 in tRNA Escherichia coli SN01 tRNA modifying enzyme S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.35 Escherichia coli
-
-
-
2.1.1.35 Escherichia coli
-
 MRE 600
-
2.1.1.35 Escherichia coli MRE 600
-
 MRE 600
-
2.1.1.35 Escherichia coli SN01
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.35 polyethyleneimine precipitation, phosphocellulose and affinity chromatography Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.35 0.0104
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.1.1.35 -70°C, at least 6 months, stable Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.35 additional information poor acceptors: tRNAs from spinach chloroplasts, Dictyostelium discoideum vegetative stage, Bacillus subtilis and Anacystis nidulans, unfractionated E. coli tRNA Escherichia coli additional information
-
 ?
2.1.1.35 additional information poor acceptors: tRNAs from spinach chloroplasts, Dictyostelium discoideum vegetative stage, Bacillus subtilis and Anacystis nidulans, unfractionated E. coli tRNA Escherichia coli MRE 600 additional information
-
 ?
2.1.1.35 additional information poor acceptors: tRNAs from spinach chloroplasts, Dictyostelium discoideum vegetative stage, Bacillus subtilis and Anacystis nidulans, unfractionated E. coli tRNA Escherichia coli SN01 additional information
-
 ?
2.1.1.35 S-adenosyl-L-methionine + uracil54 in wheat germ tRNA1-Gly
-
 Escherichia coli S-adenosyl-L-homocysteine + 5-methyluracil54 in in wheat germ tRNA1-Gly
-
 ?
2.1.1.35 S-adenosyl-L-methionine + uracil54 in wheat germ tRNA1-Gly
-
 Escherichia coli MRE 600 S-adenosyl-L-homocysteine + 5-methyluracil54 in in wheat germ tRNA1-Gly
-
 ?
2.1.1.35 S-adenosyl-L-methionine + uracil54 in wheat germ tRNA1-Gly
-
 Escherichia coli SN01 S-adenosyl-L-homocysteine + 5-methyluracil54 in in wheat germ tRNA1-Gly
-
 ?
2.1.1.35 S-adenosyl-L-methionine + uridine54 in tRNA tRNAs from wheat germ are the best substrates Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA formation of 5-methyluridine ?
2.1.1.35 S-adenosyl-L-methionine + uridine54 in tRNA methyl group acceptors: a variety of tRNA substrates including prokaryotic, eukaryotic, mitochondrial and chloroplastic tRNAs, wheat germ tRNA1Gly, bovine liver tRNAVal, Dictyostelium discoideum vegetative stage mitochondrial tRNA and rabbit liver tRNA, methylates E. coli tRNAs at a specific U residue 23 nucleotides from the 3’ end Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA formation of 5-methyluridine ?
2.1.1.35 S-adenosyl-L-methionine + uridine54 in tRNA tRNA modifying enzyme Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
 ?
2.1.1.35 S-adenosyl-L-methionine + uridine54 in tRNA tRNAs from wheat germ are the best substrates Escherichia coli MRE 600 S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA formation of 5-methyluridine ?
2.1.1.35 S-adenosyl-L-methionine + uridine54 in tRNA methyl group acceptors: a variety of tRNA substrates including prokaryotic, eukaryotic, mitochondrial and chloroplastic tRNAs, wheat germ tRNA1Gly, bovine liver tRNAVal, Dictyostelium discoideum vegetative stage mitochondrial tRNA and rabbit liver tRNA, methylates E. coli tRNAs at a specific U residue 23 nucleotides from the 3’ end Escherichia coli MRE 600 S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA formation of 5-methyluridine ?
2.1.1.35 S-adenosyl-L-methionine + uridine54 in tRNA tRNA modifying enzyme Escherichia coli MRE 600 S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
 ?
2.1.1.35 S-adenosyl-L-methionine + uridine54 in tRNA tRNAs from wheat germ are the best substrates Escherichia coli SN01 S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA formation of 5-methyluridine ?
2.1.1.35 S-adenosyl-L-methionine + uridine54 in tRNA methyl group acceptors: a variety of tRNA substrates including prokaryotic, eukaryotic, mitochondrial and chloroplastic tRNAs, wheat germ tRNA1Gly, bovine liver tRNAVal, Dictyostelium discoideum vegetative stage mitochondrial tRNA and rabbit liver tRNA, methylates E. coli tRNAs at a specific U residue 23 nucleotides from the 3’ end Escherichia coli SN01 S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA formation of 5-methyluridine ?
2.1.1.35 S-adenosyl-L-methionine + uridine54 in tRNA tRNA modifying enzyme Escherichia coli SN01 S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.35 monomer 1 * 42000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.35 30
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.35 additional information
-
 pI: 4.8, Escherichia coli MRE 600 Escherichia coli
2.1.1.35 8.4
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.35 7.8 8.8 25% inhibition of enzyme activity at pH 7.8 and 8.8 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.35 S-adenosyl-L-methionine
-
 Escherichia coli