BRENDA - Enzyme Database show

Amine N-methyltransferases from rabbit liver

Ansher, S.S.; Jakoby, W.B.; J. Biol. Chem. 261, 3996-4001 (1986)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.49
additional information
substrate inhibition, but not by tryptamine and 7,8-dichloro-1,2,3,4-tetrahydroisoquinoline
Oryctolagus cuniculus
2.1.1.49
N,N-Dimethyltryptamine
competitive inhibitor
Oryctolagus cuniculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.49
0.01
-
7,8-dichloro-1,2,3,4-tetrahydroisoquinoline
transferase A, pH 7.6
Oryctolagus cuniculus
2.1.1.49
0.015
-
S-adenosyl-L-methionine
1 mM tryptamine as methyl acceptor, pH 8.5
Oryctolagus cuniculus
2.1.1.49
0.12
-
tryptamine
transferase B, pH 8.5
Oryctolagus cuniculus
2.1.1.49
0.2
-
imidazole
transferase A, pH 8.5
Oryctolagus cuniculus
2.1.1.49
0.24
-
desmethylimipramine
transferase B, pH 8.5
Oryctolagus cuniculus
2.1.1.49
0.3
-
desmethylimipramine
transferase A, pH 8.5
Oryctolagus cuniculus
2.1.1.49
0.3
-
tryptamine
transferase A, pH 8.5
Oryctolagus cuniculus
2.1.1.49
1.5
-
pyrazole
transferase A, pH 7.6
Oryctolagus cuniculus
2.1.1.49
2
-
7,8-dichloro-1,2,3,4-tetrahydroisoquinoline
transferase B, pH 7.6
Oryctolagus cuniculus
2.1.1.49
3.2
-
pyrazole
transferase B, pH 7.6
Oryctolagus cuniculus
2.1.1.49
5.1
-
imidazole
transferase B, pH 8.5
Oryctolagus cuniculus
2.1.1.49
7.6
-
aniline
transferase A, pH 7.6
Oryctolagus cuniculus
2.1.1.49
7.8
-
aniline
transferase B, pH 7.6
Oryctolagus cuniculus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.49
additional information
-
amino acid composition of transferase A
Oryctolagus cuniculus
2.1.1.49
30000
-
gel filtration
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.49
S-adenosyl-L-methionine + an amine
Oryctolagus cuniculus
enzymes participate in detoxication, recycling of drugs after initial demethylation
S-adenosyl-L-homocysteine + an N-methylamine
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.49
Oryctolagus cuniculus
-
10-16 week old; amine N-methyltransferase A and B
-
2.1.1.49
Oryctolagus cuniculus New Zealand
-
10-16 week old
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.1.49
two N-methyltransferases A and B separable by ammonium sulfate fractionation, HPLC-ion exchange chromatography
Oryctolagus cuniculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.49
liver
two amine N-methyltransferases, A and B
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.1.49
0.000068
-
N-methyltransferase A, aniline
Oryctolagus cuniculus
2.1.1.49
0.00022
-
N-methyltransferase B, desmethylimipramine
Oryctolagus cuniculus
2.1.1.49
0.00026
-
N-methyltransferase A, desmethylimipramine
Oryctolagus cuniculus
2.1.1.49
0.00044
-
N-methyltransferase A, tryptamine
Oryctolagus cuniculus
2.1.1.49
0.00127
-
N-methyltransferase B, aniline
Oryctolagus cuniculus
2.1.1.49
0.0031
-
N-methyltransferase B, tryptamine
Oryctolagus cuniculus
Storage Stability
EC Number
Storage Stability
Organism
2.1.1.49
-80°C, partially purified, per month, less than 10% loss of activity
Oryctolagus cuniculus
2.1.1.49
-80°C, purified, per month, about 25% loss of activity
Oryctolagus cuniculus
2.1.1.49
2°C, per week, 25% loss of activity
Oryctolagus cuniculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.49
additional information
two N-methyltransferases A and B with very broad but overlapping substrate specificity for primary and secondary amines, lipophilic substrates, overview poor substrates, no substrates: N-acetylserotonine, 4-aminoacetophenone, 4-aminophenol, benzamide, 4-chloroaniline, deoxyepinephrine, 1,4-diaminobutane, dopamine, 1-ethanol-2-amine, ethylenediamine, histamine, histidine methylester, 3-hydroxytryptamine, indole, pyrrole, methylamine, N-methyldopamine, nicotinamide, octopamine, phenylalanine, phenylethanolamine, theophylline, tryptophan, tyrosine, N,N-dimethyltryptamine
441354
Oryctolagus cuniculus
?
-
-
-
-
2.1.1.49
additional information
two N-methyltransferases A and B with very broad but overlapping substrate specificity for primary and secondary amines, lipophilic substrates, overview poor substrates, no substrates: N-acetylserotonine, 4-aminoacetophenone, 4-aminophenol, benzamide, 4-chloroaniline, deoxyepinephrine, 1,4-diaminobutane, dopamine, 1-ethanol-2-amine, ethylenediamine, histamine, histidine methylester, 3-hydroxytryptamine, indole, pyrrole, methylamine, N-methyldopamine, nicotinamide, octopamine, phenylalanine, phenylethanolamine, theophylline, tryptophan, tyrosine, N,N-dimethyltryptamine
441354
Oryctolagus cuniculus New Zealand
?
-
-
-
-
2.1.1.49
S-adenosyl-L-methionine + 5-methyloxytryptamine
higher activity with transferase A
441354
Oryctolagus cuniculus
?
-
-
-
?
2.1.1.49
S-adenosyl-L-methionine + 7,8-dichloro-1,2,3,4-tetrahydroisoquinoline
substrate with highest apparent kcat
441354
Oryctolagus cuniculus
?
-
-
-
?
2.1.1.49
S-adenosyl-L-methionine + alpha-N-methyltryptamine
-
441354
Oryctolagus cuniculus
S-adenosyl-L-homocysteine + alpha,alpha-N,N-dimethyltryptamine
-
-
-
?
2.1.1.49
S-adenosyl-L-methionine + alpha-N-methyltryptamine
-
441354
Oryctolagus cuniculus New Zealand
S-adenosyl-L-homocysteine + alpha,alpha-N,N-dimethyltryptamine
-
-
-
?
2.1.1.49
S-adenosyl-L-methionine + an amine
enzymes participate in detoxication, recycling of drugs after initial demethylation
441354
Oryctolagus cuniculus
S-adenosyl-L-homocysteine + an N-methylamine
-
-
-
?
2.1.1.49
S-adenosyl-L-methionine + aniline
poor substrate
441354
Oryctolagus cuniculus
S-adenosyl-L-homocysteine + N-methylaniline
-
-
-
?
2.1.1.49
S-adenosyl-L-methionine + anisidine
higher activity with transferase B
441354
Oryctolagus cuniculus
?
-
-
-
?
2.1.1.49
S-adenosyl-L-methionine + desmethylimipramine
higher activity with transferase A
441354
Oryctolagus cuniculus
S-adenosyl-L-homocysteine + imipramine
-
-
-
?
2.1.1.49
S-adenosyl-L-methionine + desmethylimipramine
higher activity with transferase A
441354
Oryctolagus cuniculus New Zealand
S-adenosyl-L-homocysteine + imipramine
-
-
-
?
2.1.1.49
S-adenosyl-L-methionine + imidazole
higher activity with transferase A
441354
Oryctolagus cuniculus
?
-
-
-
?
2.1.1.49
S-adenosyl-L-methionine + N-methylbutylamine
only transferase A
441354
Oryctolagus cuniculus
S-adenosyl-L-homocysteine + N,N-dimethylbutylamine
-
-
-
?
2.1.1.49
S-adenosyl-L-methionine + N-methylcyclohexylamine
only transferase A
441354
Oryctolagus cuniculus
S-adenosyl-L-homocysteine + N,N-dimethylcyclohexylamine
-
-
-
?
2.1.1.49
S-adenosyl-L-methionine + N-methyltryptamine
-
441354
Oryctolagus cuniculus
S-adenosyl-L-homocysteine + N,N-dimethyltryptamine
-
-
-
?
2.1.1.49
S-adenosyl-L-methionine + N-methyltryptamine
-
441354
Oryctolagus cuniculus New Zealand
S-adenosyl-L-homocysteine + N,N-dimethyltryptamine
-
-
-
?
2.1.1.49
S-adenosyl-L-methionine + phenyltetrahydropyridine
higher activity with transferase A
441354
Oryctolagus cuniculus
?
-
-
-
?
2.1.1.49
S-adenosyl-L-methionine + pyrazole
higher activity with transferase A
441354
Oryctolagus cuniculus
?
-
-
-
?
2.1.1.49
S-adenosyl-L-methionine + tryptamine
i.e. 3-[2-aminoethyl] indole, best substrate, two N-methyltransferases: A and B, non-specific
441354
Oryctolagus cuniculus
S-adenosyl-L-homocysteine + alpha-N-methyltryptamine
-
441354
Oryctolagus cuniculus
?
2.1.1.49
S-adenosyl-L-methionine + tryptamine
i.e. 3-[2-aminoethyl] indole, best substrate, two N-methyltransferases: A and B, non-specific
441354
Oryctolagus cuniculus New Zealand
S-adenosyl-L-homocysteine + alpha-N-methyltryptamine
-
441354
Oryctolagus cuniculus New Zealand
?
Subunits
EC Number
Subunits
Commentary
Organism
2.1.1.49
monomer
1 * 30000, SDS-PAGE
Oryctolagus cuniculus
2.1.1.49
More
aggregation upon denaturation leads to 60 kDa and 90 kDa fractions
Oryctolagus cuniculus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.1.1.49
37
-
assay at
Oryctolagus cuniculus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.1.1.49
0.0333
-
aniline
transferase A, pH 7.6
Oryctolagus cuniculus
2.1.1.49
0.0333
-
desmethylimipramine
transferase B, pH 8.5
Oryctolagus cuniculus
2.1.1.49
0.0833
-
imidazole
transferase A, pH 8.5
Oryctolagus cuniculus
2.1.1.49
0.1
-
pyrazole
transferase B, pH 7.6
Oryctolagus cuniculus
2.1.1.49
0.117
-
S-adenosyl-L-methionine
transferase B, pH 8.5
Oryctolagus cuniculus
2.1.1.49
0.167
-
imidazole
transferase B, pH 8.5
Oryctolagus cuniculus
2.1.1.49
0.2
-
S-adenosyl-L-methionine
transferase A, pH 8.5
Oryctolagus cuniculus
2.1.1.49
0.233
-
desmethylimipramine
transferase A, pH 8.5
Oryctolagus cuniculus
2.1.1.49
0.25
-
pyrazole
transferase A, pH 7.6
Oryctolagus cuniculus
2.1.1.49
0.367
-
aniline
transferase B, pH 7.6
Oryctolagus cuniculus
2.1.1.49
0.4
-
tryptamine
transferase A, pH 8.5
Oryctolagus cuniculus
2.1.1.49
0.45
-
tryptamine
transferase B, pH 8.5
Oryctolagus cuniculus
2.1.1.49
0.667
-
7,8-dichloro-1,2,3,4-tetrahydroisoquinoline
transferase A, pH 7.6
Oryctolagus cuniculus
2.1.1.49
3.67
-
7,8-dichloro-1,2,3,4-tetrahydroisoquinoline
transferase B, pH 7.6
Oryctolagus cuniculus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.49
additional information
-
transferase A: pI 4.9, transferase B: pI 5.1
Oryctolagus cuniculus
2.1.1.49
8.5
-
-
Oryctolagus cuniculus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.1.1.49
7.2
8.9
tryptamine, about half-maximal activity at pH 7.2 and 8.9, transferase A
Oryctolagus cuniculus
2.1.1.49
7.5
8.8
tryptamine, about half-maximal activity at pH 7.5 and 90% at pH 8.8, transferase B
Oryctolagus cuniculus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.1.1.49
6.2
-
optimal for transferase A
Oryctolagus cuniculus
2.1.1.49
7.8
-
optimal for transferase B
Oryctolagus cuniculus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.49
S-adenosyl-L-methionine
-
Oryctolagus cuniculus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.1.1.49
0.006
-
N,N-Dimethyltryptamine
N-methyltransferase B
Oryctolagus cuniculus
2.1.1.49
0.01
-
N,N-Dimethyltryptamine
N-methyltransferase A
Oryctolagus cuniculus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.49
S-adenosyl-L-methionine
-
Oryctolagus cuniculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.49
additional information
substrate inhibition, but not by tryptamine and 7,8-dichloro-1,2,3,4-tetrahydroisoquinoline
Oryctolagus cuniculus
2.1.1.49
N,N-Dimethyltryptamine
competitive inhibitor
Oryctolagus cuniculus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.1.1.49
0.006
-
N,N-Dimethyltryptamine
N-methyltransferase B
Oryctolagus cuniculus
2.1.1.49
0.01
-
N,N-Dimethyltryptamine
N-methyltransferase A
Oryctolagus cuniculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.49
0.01
-
7,8-dichloro-1,2,3,4-tetrahydroisoquinoline
transferase A, pH 7.6
Oryctolagus cuniculus
2.1.1.49
0.015
-
S-adenosyl-L-methionine
1 mM tryptamine as methyl acceptor, pH 8.5
Oryctolagus cuniculus
2.1.1.49
0.12
-
tryptamine
transferase B, pH 8.5
Oryctolagus cuniculus
2.1.1.49
0.2
-
imidazole
transferase A, pH 8.5
Oryctolagus cuniculus
2.1.1.49
0.24
-
desmethylimipramine
transferase B, pH 8.5
Oryctolagus cuniculus
2.1.1.49
0.3
-
desmethylimipramine
transferase A, pH 8.5
Oryctolagus cuniculus
2.1.1.49
0.3
-
tryptamine
transferase A, pH 8.5
Oryctolagus cuniculus
2.1.1.49
1.5
-
pyrazole
transferase A, pH 7.6
Oryctolagus cuniculus
2.1.1.49
2
-
7,8-dichloro-1,2,3,4-tetrahydroisoquinoline
transferase B, pH 7.6
Oryctolagus cuniculus
2.1.1.49
3.2
-
pyrazole
transferase B, pH 7.6
Oryctolagus cuniculus
2.1.1.49
5.1
-
imidazole
transferase B, pH 8.5
Oryctolagus cuniculus
2.1.1.49
7.6
-
aniline
transferase A, pH 7.6
Oryctolagus cuniculus
2.1.1.49
7.8
-
aniline
transferase B, pH 7.6
Oryctolagus cuniculus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.49
additional information
-
amino acid composition of transferase A
Oryctolagus cuniculus
2.1.1.49
30000
-
gel filtration
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.49
S-adenosyl-L-methionine + an amine
Oryctolagus cuniculus
enzymes participate in detoxication, recycling of drugs after initial demethylation
S-adenosyl-L-homocysteine + an N-methylamine
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.49
two N-methyltransferases A and B separable by ammonium sulfate fractionation, HPLC-ion exchange chromatography
Oryctolagus cuniculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.49
liver
two amine N-methyltransferases, A and B
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.1.49
0.000068
-
N-methyltransferase A, aniline
Oryctolagus cuniculus
2.1.1.49
0.00022
-
N-methyltransferase B, desmethylimipramine
Oryctolagus cuniculus
2.1.1.49
0.00026
-
N-methyltransferase A, desmethylimipramine
Oryctolagus cuniculus
2.1.1.49
0.00044
-
N-methyltransferase A, tryptamine
Oryctolagus cuniculus
2.1.1.49
0.00127
-
N-methyltransferase B, aniline
Oryctolagus cuniculus
2.1.1.49
0.0031
-
N-methyltransferase B, tryptamine
Oryctolagus cuniculus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.1.1.49
-80°C, partially purified, per month, less than 10% loss of activity
Oryctolagus cuniculus
2.1.1.49
-80°C, purified, per month, about 25% loss of activity
Oryctolagus cuniculus
2.1.1.49
2°C, per week, 25% loss of activity
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.49
additional information
two N-methyltransferases A and B with very broad but overlapping substrate specificity for primary and secondary amines, lipophilic substrates, overview poor substrates, no substrates: N-acetylserotonine, 4-aminoacetophenone, 4-aminophenol, benzamide, 4-chloroaniline, deoxyepinephrine, 1,4-diaminobutane, dopamine, 1-ethanol-2-amine, ethylenediamine, histamine, histidine methylester, 3-hydroxytryptamine, indole, pyrrole, methylamine, N-methyldopamine, nicotinamide, octopamine, phenylalanine, phenylethanolamine, theophylline, tryptophan, tyrosine, N,N-dimethyltryptamine
441354
Oryctolagus cuniculus
?
-
-
-
-
2.1.1.49
additional information
two N-methyltransferases A and B with very broad but overlapping substrate specificity for primary and secondary amines, lipophilic substrates, overview poor substrates, no substrates: N-acetylserotonine, 4-aminoacetophenone, 4-aminophenol, benzamide, 4-chloroaniline, deoxyepinephrine, 1,4-diaminobutane, dopamine, 1-ethanol-2-amine, ethylenediamine, histamine, histidine methylester, 3-hydroxytryptamine, indole, pyrrole, methylamine, N-methyldopamine, nicotinamide, octopamine, phenylalanine, phenylethanolamine, theophylline, tryptophan, tyrosine, N,N-dimethyltryptamine
441354
Oryctolagus cuniculus New Zealand
?
-
-
-
-
2.1.1.49
S-adenosyl-L-methionine + 5-methyloxytryptamine
higher activity with transferase A
441354
Oryctolagus cuniculus
?
-
-
-
?
2.1.1.49
S-adenosyl-L-methionine + 7,8-dichloro-1,2,3,4-tetrahydroisoquinoline
substrate with highest apparent kcat
441354
Oryctolagus cuniculus
?
-
-
-
?
2.1.1.49
S-adenosyl-L-methionine + alpha-N-methyltryptamine
-
441354
Oryctolagus cuniculus
S-adenosyl-L-homocysteine + alpha,alpha-N,N-dimethyltryptamine
-
-
-
?
2.1.1.49
S-adenosyl-L-methionine + alpha-N-methyltryptamine
-
441354
Oryctolagus cuniculus New Zealand
S-adenosyl-L-homocysteine + alpha,alpha-N,N-dimethyltryptamine
-
-
-
?
2.1.1.49
S-adenosyl-L-methionine + an amine
enzymes participate in detoxication, recycling of drugs after initial demethylation
441354
Oryctolagus cuniculus
S-adenosyl-L-homocysteine + an N-methylamine
-
-
-
?
2.1.1.49
S-adenosyl-L-methionine + aniline
poor substrate
441354
Oryctolagus cuniculus
S-adenosyl-L-homocysteine + N-methylaniline
-
-
-
?
2.1.1.49
S-adenosyl-L-methionine + anisidine
higher activity with transferase B
441354
Oryctolagus cuniculus
?
-
-
-
?
2.1.1.49
S-adenosyl-L-methionine + desmethylimipramine
higher activity with transferase A
441354
Oryctolagus cuniculus
S-adenosyl-L-homocysteine + imipramine
-
-
-
?
2.1.1.49
S-adenosyl-L-methionine + desmethylimipramine
higher activity with transferase A
441354
Oryctolagus cuniculus New Zealand
S-adenosyl-L-homocysteine + imipramine
-
-
-
?
2.1.1.49
S-adenosyl-L-methionine + imidazole
higher activity with transferase A
441354
Oryctolagus cuniculus
?
-
-
-
?
2.1.1.49
S-adenosyl-L-methionine + N-methylbutylamine
only transferase A
441354
Oryctolagus cuniculus
S-adenosyl-L-homocysteine + N,N-dimethylbutylamine
-
-
-
?
2.1.1.49
S-adenosyl-L-methionine + N-methylcyclohexylamine
only transferase A
441354
Oryctolagus cuniculus
S-adenosyl-L-homocysteine + N,N-dimethylcyclohexylamine
-
-
-
?
2.1.1.49
S-adenosyl-L-methionine + N-methyltryptamine
-
441354
Oryctolagus cuniculus
S-adenosyl-L-homocysteine + N,N-dimethyltryptamine
-
-
-
?
2.1.1.49
S-adenosyl-L-methionine + N-methyltryptamine
-
441354
Oryctolagus cuniculus New Zealand
S-adenosyl-L-homocysteine + N,N-dimethyltryptamine
-
-
-
?
2.1.1.49
S-adenosyl-L-methionine + phenyltetrahydropyridine
higher activity with transferase A
441354
Oryctolagus cuniculus
?
-
-
-
?
2.1.1.49
S-adenosyl-L-methionine + pyrazole
higher activity with transferase A
441354
Oryctolagus cuniculus
?
-
-
-
?
2.1.1.49
S-adenosyl-L-methionine + tryptamine
i.e. 3-[2-aminoethyl] indole, best substrate, two N-methyltransferases: A and B, non-specific
441354
Oryctolagus cuniculus
S-adenosyl-L-homocysteine + alpha-N-methyltryptamine
-
441354
Oryctolagus cuniculus
?
2.1.1.49
S-adenosyl-L-methionine + tryptamine
i.e. 3-[2-aminoethyl] indole, best substrate, two N-methyltransferases: A and B, non-specific
441354
Oryctolagus cuniculus New Zealand
S-adenosyl-L-homocysteine + alpha-N-methyltryptamine
-
441354
Oryctolagus cuniculus New Zealand
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.1.1.49
monomer
1 * 30000, SDS-PAGE
Oryctolagus cuniculus
2.1.1.49
More
aggregation upon denaturation leads to 60 kDa and 90 kDa fractions
Oryctolagus cuniculus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.1.1.49
37
-
assay at
Oryctolagus cuniculus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.1.1.49
0.0333
-
aniline
transferase A, pH 7.6
Oryctolagus cuniculus
2.1.1.49
0.0333
-
desmethylimipramine
transferase B, pH 8.5
Oryctolagus cuniculus
2.1.1.49
0.0833
-
imidazole
transferase A, pH 8.5
Oryctolagus cuniculus
2.1.1.49
0.1
-
pyrazole
transferase B, pH 7.6
Oryctolagus cuniculus
2.1.1.49
0.117
-
S-adenosyl-L-methionine
transferase B, pH 8.5
Oryctolagus cuniculus
2.1.1.49
0.167
-
imidazole
transferase B, pH 8.5
Oryctolagus cuniculus
2.1.1.49
0.2
-
S-adenosyl-L-methionine
transferase A, pH 8.5
Oryctolagus cuniculus
2.1.1.49
0.233
-
desmethylimipramine
transferase A, pH 8.5
Oryctolagus cuniculus
2.1.1.49
0.25
-
pyrazole
transferase A, pH 7.6
Oryctolagus cuniculus
2.1.1.49
0.367
-
aniline
transferase B, pH 7.6
Oryctolagus cuniculus
2.1.1.49
0.4
-
tryptamine
transferase A, pH 8.5
Oryctolagus cuniculus
2.1.1.49
0.45
-
tryptamine
transferase B, pH 8.5
Oryctolagus cuniculus
2.1.1.49
0.667
-
7,8-dichloro-1,2,3,4-tetrahydroisoquinoline
transferase A, pH 7.6
Oryctolagus cuniculus
2.1.1.49
3.67
-
7,8-dichloro-1,2,3,4-tetrahydroisoquinoline
transferase B, pH 7.6
Oryctolagus cuniculus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.49
additional information
-
transferase A: pI 4.9, transferase B: pI 5.1
Oryctolagus cuniculus
2.1.1.49
8.5
-
-
Oryctolagus cuniculus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.1.1.49
7.2
8.9
tryptamine, about half-maximal activity at pH 7.2 and 8.9, transferase A
Oryctolagus cuniculus
2.1.1.49
7.5
8.8
tryptamine, about half-maximal activity at pH 7.5 and 90% at pH 8.8, transferase B
Oryctolagus cuniculus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.1.1.49
6.2
-
optimal for transferase A
Oryctolagus cuniculus
2.1.1.49
7.8
-
optimal for transferase B
Oryctolagus cuniculus