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Literature summary extracted from

  • Hurwitz, J.; Gold, M.; Anders, M.
    The enzymatic methylation of ribonucleic acid and deoxyribonucleic acid. IV. The properties of the soluble ribonucleic acid-methylating enzymes (1964), J. Biol. Chem., 239, 3474-3482.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.33 2-mercaptoethanol activates Escherichia coli
2.1.1.33 additional information not activated by spermine Escherichia coli
2.1.1.35 2-mercaptoethanol activates Escherichia coli
2.1.1.35 spermine slight activation Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.33 additional information not inhibited by adenosine, L-homocysteine, snake venom phosphodiesterase Escherichia coli
2.1.1.33 p-hydroxymercuribenzoate
-
Escherichia coli
2.1.1.33 S-adenosyl-L-homocysteine product inhibition, reversed by S-adenosylmethionine Escherichia coli
2.1.1.33 Spleen phosphodiesterase
-
Escherichia coli
2.1.1.35 additional information not inhibited by spermine, adenosine, L-homocysteine, snake venom phosphodiesterase Escherichia coli
2.1.1.35 Ni2+
-
Escherichia coli
2.1.1.35 p-hydroxymercuribenzoate
-
Escherichia coli
2.1.1.35 ribonuclease inactivates Escherichia coli
2.1.1.35 S-adenosyl-L-homocysteine product inhibition, reversed by S-adenosylmethionine Escherichia coli
2.1.1.35 Spleen phosphodiesterase weak Escherichia coli
2.1.1.35 Zn2+
-
Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.33 0.016
-
S-adenosyl-L-methionine
-
Escherichia coli
2.1.1.33 0.21
-
methyl deficient tRNA from E. coli
-
Escherichia coli
2.1.1.35 0.018
-
S-adenosyl-L-methionine
-
Escherichia coli
2.1.1.35 0.29
-
uracil54 in methyl deficient tRNA from E. coli
-
Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.33 soluble
-
Escherichia coli
-
-
2.1.1.35 soluble
-
Escherichia coli
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.33 Ca2+ activates Escherichia coli
2.1.1.33 Mg2+ in presence of Mg2+ 2fold increase of activity Escherichia coli
2.1.1.33 Mn2+ activates Escherichia coli
2.1.1.35 Ca2+ activates Escherichia coli
2.1.1.35 Mg2+ in presence of Mg2+ 4fold increase of activity Escherichia coli
2.1.1.35 Mn2+ activates Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.33 Escherichia coli
-
-
-
2.1.1.35 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.33
-
Escherichia coli
2.1.1.35
-
Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.35 0.0064
-
-
Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.33 additional information not as methyl group acceptors: methyl-deficient DNA from E. coli, polyadenylic acid, polycytidylic acid, polyuridylic acid, polyinosinic acid, copolymers of adenylic acid and uridylic acid and of guanylic acid and cytidylic acid, tobacco mosaic viral RNA, turnip yellow mosaic viral RNA, RNA from bacteriophage f2, CMP, AMP, UMP, GMP, rRNA Escherichia coli additional information
-
?
2.1.1.33 S-adenosyl-L-methionine + guanine46 in methyl deficient tRNA from E. coli
-
Escherichia coli ?
-
?
2.1.1.33 S-adenosyl-L-methionine + guanine46 in tRNA methyl group acceptor: methyl-deficient tRNA from E. coli Escherichia coli S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
ir
2.1.1.33 S-adenosyl-L-methionine + guanine46 in tRNA highly specific for tRNA and S-adenosyl-L-methionine, cannot be replaced by methionine plus ATP, formaldehyde plus tetrahydrofolic acid, methylcobalamin and methyltetrahydrofolic acid Escherichia coli S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
ir
2.1.1.33 S-adenosyl-L-methionine + guanine46 in tRNA extend of methylation is directly proportional to the amount of tRNA added, 0.19% of nucleotide residues are methylated Escherichia coli S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
ir
2.1.1.35 additional information not as methyl group acceptors: methyl-deficient DNA from E. coli, polyadenylic acid, polycytidylic acid, polyuridylic acid, polyinosinic acid, copolymers of adenylic acid and uridylic acid and of guanylic acid and cytidylic acid, tobacco mosaic viral RNA, turnip yellow mosaic viral RNA, RNA from bacteriophage f2, CMP, AMP, UMP, GMP, rRNA Escherichia coli additional information
-
?
2.1.1.35 S-adenosyl-L-methionine + uracil54 in methyl deficient tRNA from E. coli
-
Escherichia coli ?
-
?
2.1.1.35 S-adenosyl-L-methionine + uridine54 in tRNA methyl group acceptor: methyl-deficient tRNA from E. coli Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
ir
2.1.1.35 S-adenosyl-L-methionine + uridine54 in tRNA highly specific for tRNA and S-adenosyl-L-methionine, cannot be replaced by methionine plus ATP, formaldehyde plus tetrahydrofolic acid, methylcobalamin and methyltetrahydrofolic acid Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
ir
2.1.1.35 S-adenosyl-L-methionine + uridine54 in tRNA extend of methylation is directly proportional to the amount of tRNA added, 0.5% of nucleotide residues are methylated Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
ir

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.33 7.5 8 dimethylglutarate buffer Escherichia coli
2.1.1.35 8.5 9 triethanolamine buffer Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.33 S-adenosyl-L-methionine
-
Escherichia coli
2.1.1.35 S-adenosyl-L-methionine
-
Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.35 0.02
-
S-adenosyl-L-homocysteine
-
Escherichia coli