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Literature summary extracted from

  • Hurwitz, J.; Gold, M.; Anders, M.
    The enzymatic methylation of ribonucleic acid and deoxyribonucleic acid. III. Purification of soluble ribonucleic acid-methylating enzymes (1964), J. Biol. Chem., 239, 3462-3473.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.33 soluble
-
 Escherichia coli
-
-
2.1.1.35 soluble
-
 Escherichia coli
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.33 Escherichia coli
-
-
-
2.1.1.35 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.33 partial Escherichia coli
2.1.1.35 partial Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.35 0.0051
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.1.1.33 0°C, partially purified, 48 h, 15% loss of activity Escherichia coli
2.1.1.33 0°C, purified, 6 months, about 70% loss of activity Escherichia coli
2.1.1.35 0°C, partially purified, 48 h, about 30% loss of activity Escherichia coli
2.1.1.35 0°C, purified, 6 months, stable Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.33 S-adenosyl-L-methionine + guanine46 in tRNA methyl group acceptor: methyl-deficient tRNA from E. coli Escherichia coli S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
 ?
2.1.1.35 S-adenosyl-L-methionine + uridine54 in tRNA methyl group acceptor: methyl-deficient tRNA from E. coli Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
 ?
2.1.1.35 S-adenosyl-L-methionine + uridine54 in tRNA methyl group acceptor: tRNA from Micrococcus lysodeikticus Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.33 38
-
 assay at Escherichia coli
2.1.1.35 38
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.33 S-adenosyl-L-methionine
-
 Escherichia coli
2.1.1.35 S-adenosyl-L-methionine
-
 Escherichia coli