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Literature summary extracted from

  • Eichel, J.; Gonzalez, J.C.; Hotze, M.; Matthews, R.G.; Schroeder, J.
    Vitamin-B12-independent methionine synthase from a higher plant (Catharanthus roseus). Molecular characterization, regulation, heterologous expression, and enzyme properties (1995), Eur. J. Biochem., 230, 1053-1058.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.14
-
 Catharanthus roseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.14 0.01
-
 L-homocysteine or less Catharanthus roseus
2.1.1.14 0.028
-
 5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate
-
 Catharanthus roseus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.14 cytosol
-
 Catharanthus roseus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.14 85000
-
 x * 85000, deduced from DNA sequence, SDS-PAGE of recombinant protein Catharanthus roseus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.14 Catharanthus roseus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.14 5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
 Catharanthus roseus tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.14 ? x * 85000, deduced from DNA sequence, SDS-PAGE of recombinant protein Catharanthus roseus

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.14 additional information no requirement for phosphate or magnesium Catharanthus roseus
2.1.1.14 additional information vitamin-B12 independent enzyme Catharanthus roseus
2.1.1.14 additional information no requirement for S-adenosylmethionine Catharanthus roseus