BRENDA - Enzyme Database

Vitamin-B12-independent methionine synthase from a higher plant (Catharanthus roseus). Molecular characterization, regulation, heterologous expression, and enzyme properties

Eichel, J.; Gonzalez, J.C.; Hotze, M.; Matthews, R.G.; Schroeder, J.; Eur. J. Biochem. 230, 1053-1058 (1995)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.1.1.14
-
Catharanthus roseus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.14
0.01
-
L-homocysteine
or less
Catharanthus roseus
2.1.1.14
0.028
-
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate
-
Catharanthus roseus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.1.14
cytosol
-
Catharanthus roseus
5829
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.14
85000
-
x * 85000, deduced from DNA sequence, SDS-PAGE of recombinant protein
Catharanthus roseus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.14
Catharanthus roseus
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.14
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
441331
Catharanthus roseus
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.1.1.14
?
x * 85000, deduced from DNA sequence, SDS-PAGE of recombinant protein
Catharanthus roseus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.14
additional information
no requirement for phosphate or magnesium; no requirement for S-adenosylmethionine; vitamin-B12 independent enzyme
Catharanthus roseus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.14
-
Catharanthus roseus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.14
additional information
no requirement for phosphate or magnesium; no requirement for S-adenosylmethionine; vitamin-B12 independent enzyme
Catharanthus roseus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.14
0.01
-
L-homocysteine
or less
Catharanthus roseus
2.1.1.14
0.028
-
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate
-
Catharanthus roseus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.1.14
cytosol
-
Catharanthus roseus
5829
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.14
85000
-
x * 85000, deduced from DNA sequence, SDS-PAGE of recombinant protein
Catharanthus roseus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.14
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
441331
Catharanthus roseus
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.1.1.14
?
x * 85000, deduced from DNA sequence, SDS-PAGE of recombinant protein
Catharanthus roseus