BRENDA - Enzyme Database

Purification and properties of 5-methyltetrahydropteroyltriglutamate-homocysteine transmethylase

Whitefield, C.D.; Steers, E.J.; Weissbach, H.; J. Biol. Chem. 245, 390-401 (1970)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
2.1.1.14
-
Escherichia coli
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.14
5-methyltetrahydropteroyl-alpha-glutamate
-
Escherichia coli
2.1.1.14
EDTA
in absence of Mg2+
Escherichia coli
2.1.1.14
high ionic strength
-
Escherichia coli
2.1.1.14
Pteroyl-alpha-glutamylglutamic acid
-
Escherichia coli
2.1.1.14
Pteroyl-gamma-glutamyl-gamma-glutamylglutamic acid
-
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.14
0.0044
-
5-methyltetrahydropteroyltriglutamate
-
Escherichia coli
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.1.14
soluble
-
Escherichia coli
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.1.1.14
Mg2+
at low phosphate concentration, 0.1 mM, the Mg2+-stimulation is 4.5fold, smaller stimulation at higher concentrations of phosphate; stimulates
Escherichia coli
2.1.1.14
phosphate
-
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.14
50800
-
x * 50800, equilibrium sedimentation of carboxymethylated enzyme in 5 M guanidine-HCl
Escherichia coli
2.1.1.14
84000
-
equilibrium ultracentrifugation
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.14
Escherichia coli
-
K12
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.1.14
-
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.1.14
2.5
-
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.14
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
441323
Escherichia coli
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
-
-
?
2.1.1.14
additional information
5-methyltetrahydropteroyl-alpha-glutamate or 5-methyl-tetrahydropteroyl-alpha-glutamylglutamate cannot replace the triglutamate folate derivative as methyl donor
441323
Escherichia coli
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.1.1.14
?
x * 50800, equilibrium sedimentation of carboxymethylated enzyme in 5 M guanidine-HCl
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.14
7.5
7.8
-
Escherichia coli
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.1.1.14
6
8.5
-
Escherichia coli
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.14
additional information
vitamin-B12 independent enzyme
Escherichia coli
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.14
additional information
vitamin-B12 independent enzyme
Escherichia coli
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
2.1.1.14
-
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.14
5-methyltetrahydropteroyl-alpha-glutamate
-
Escherichia coli
2.1.1.14
EDTA
in absence of Mg2+
Escherichia coli
2.1.1.14
high ionic strength
-
Escherichia coli
2.1.1.14
Pteroyl-alpha-glutamylglutamic acid
-
Escherichia coli
2.1.1.14
Pteroyl-gamma-glutamyl-gamma-glutamylglutamic acid
-
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.14
0.0044
-
5-methyltetrahydropteroyltriglutamate
-
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.1.14
soluble
-
Escherichia coli
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.1.1.14
Mg2+
at low phosphate concentration, 0.1 mM, the Mg2+-stimulation is 4.5fold, smaller stimulation at higher concentrations of phosphate; stimulates
Escherichia coli
2.1.1.14
phosphate
-
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.14
50800
-
x * 50800, equilibrium sedimentation of carboxymethylated enzyme in 5 M guanidine-HCl
Escherichia coli
2.1.1.14
84000
-
equilibrium ultracentrifugation
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.14
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.1.14
2.5
-
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.14
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
441323
Escherichia coli
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
-
-
?
2.1.1.14
additional information
5-methyltetrahydropteroyl-alpha-glutamate or 5-methyl-tetrahydropteroyl-alpha-glutamylglutamate cannot replace the triglutamate folate derivative as methyl donor
441323
Escherichia coli
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.1.1.14
?
x * 50800, equilibrium sedimentation of carboxymethylated enzyme in 5 M guanidine-HCl
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.14
7.5
7.8
-
Escherichia coli
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.1.1.14
6
8.5
-
Escherichia coli