BRENDA - Enzyme Database

Sulphur metabolism. F. Enzymes involved in the synthesis of methionine

Madison, J.T.; Methods Plant Biochem. 3, 361-369 (1990)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.14
methionine
-
Daucus carota
2.1.1.14
methionine
-
Hordeum vulgare
2.1.1.14
methionine
-
Phaseolus sp.
2.1.1.14
methionine
-
Pisum sativum
2.1.1.14
methionine
-
Spinacia oleracea
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.14
0.000026
-
5-methyltetrahydropteroylglutamate
-
Pisum sativum
2.1.1.14
0.0022
-
S-methylmethionine
-
Pisum sativum
2.1.1.14
0.004
-
S-adenosylmethionine
-
Pisum sativum
2.1.1.14
0.041
-
homocysteine
cosubstrate S-methyl-L-methionine
Phaseolus sp.
2.1.1.14
0.055
-
S-methylmethionine
-
Phaseolus sp.
2.1.1.14
0.6
-
homocysteine
cosubstrate 5-methyltetrahydropteroylglutamate
Pisum sativum
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.1.14
chloroplast
-
Daucus carota
9507
-
2.1.1.14
chloroplast
-
Hordeum vulgare
9507
-
2.1.1.14
chloroplast
-
Phaseolus sp.
9507
-
2.1.1.14
chloroplast
-
Pisum sativum
9507
-
2.1.1.14
chloroplast
-
Spinacia oleracea
9507
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.1.1.14
Mg2+
stimulates
Daucus carota
2.1.1.14
Mg2+
stimulates
Hordeum vulgare
2.1.1.14
Mg2+
stimulates
Phaseolus sp.
2.1.1.14
Mg2+
stimulates
Pisum sativum
2.1.1.14
Mg2+
stimulates
Spinacia oleracea
2.1.1.14
phosphate
-
Daucus carota
2.1.1.14
phosphate
-
Hordeum vulgare
2.1.1.14
phosphate
-
Phaseolus sp.
2.1.1.14
phosphate
-
Pisum sativum
2.1.1.14
phosphate
-
Spinacia oleracea
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.14
additional information
Hordeum vulgare
final step in methionine synthesis
?
-
-
-
2.1.1.14
additional information
Spinacia oleracea
final step in methionine synthesis
?
-
-
-
2.1.1.14
additional information
Pisum sativum
final step in methionine synthesis
?
-
-
-
2.1.1.14
additional information
Daucus carota
final step in methionine synthesis
?
-
-
-
2.1.1.14
additional information
Phaseolus sp.
final step in methionine synthesis
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.14
Daucus carota
-
carrot
-
2.1.1.14
Hordeum vulgare
-
barley
-
2.1.1.14
Phaseolus sp.
-
bean
-
2.1.1.14
Pisum sativum
-
pea, 2 enzyme forms: a 5-methyltetrahydropteroylglutamate dependent enzyme and a S-methylmethionine dependent enzyme
-
2.1.1.14
Spinacia oleracea
-
spinach
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.1.14
-
Phaseolus sp.
Reaction
EC Number
Reaction
Commentary
Organism
2.1.1.14
5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine
final step in methionine synthesis
Daucus carota
2.1.1.14
5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine
final step in methionine synthesis
Hordeum vulgare
2.1.1.14
5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine
final step in methionine synthesis
Phaseolus sp.
2.1.1.14
5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine
final step in methionine synthesis
Pisum sativum
2.1.1.14
5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine
final step in methionine synthesis
Spinacia oleracea
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.14
root
-
Daucus carota
-
2.1.1.14
root
-
Hordeum vulgare
-
2.1.1.14
root
-
Phaseolus sp.
-
2.1.1.14
root
-
Pisum sativum
-
2.1.1.14
root
-
Spinacia oleracea
-
2.1.1.14
seedling
-
Daucus carota
-
2.1.1.14
seedling
-
Hordeum vulgare
-
2.1.1.14
seedling
-
Phaseolus sp.
-
2.1.1.14
seedling
-
Pisum sativum
-
2.1.1.14
seedling
-
Spinacia oleracea
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.14
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
441322
Hordeum vulgare
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
441322
Hordeum vulgare
?
2.1.1.14
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
441322
Spinacia oleracea
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
441322
Spinacia oleracea
?
2.1.1.14
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
441322
Pisum sativum
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
441322
Pisum sativum
?
2.1.1.14
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
441322
Daucus carota
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
441322
Daucus carota
?
2.1.1.14
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
441322
Phaseolus sp.
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
441322
Phaseolus sp.
?
2.1.1.14
additional information
enzymes of higher plants can use the monoglutamate
441322
Hordeum vulgare
?
-
-
-
-
2.1.1.14
additional information
enzymes of higher plants can use the monoglutamate
441322
Spinacia oleracea
?
-
-
-
-
2.1.1.14
additional information
enzymes of higher plants can use the monoglutamate
441322
Pisum sativum
?
-
-
-
-
2.1.1.14
additional information
enzymes of higher plants can use the monoglutamate
441322
Daucus carota
?
-
-
-
-
2.1.1.14
additional information
enzymes of higher plants can use the monoglutamate
441322
Phaseolus sp.
?
-
-
-
-
2.1.1.14
additional information
most active with the polyglutamate forms of N5-methyltetrahydropteroylglutamate
441322
Hordeum vulgare
?
-
-
-
-
2.1.1.14
additional information
most active with the polyglutamate forms of N5-methyltetrahydropteroylglutamate
441322
Spinacia oleracea
?
-
-
-
-
2.1.1.14
additional information
most active with the polyglutamate forms of N5-methyltetrahydropteroylglutamate
441322
Pisum sativum
?
-
-
-
-
2.1.1.14
additional information
most active with the polyglutamate forms of N5-methyltetrahydropteroylglutamate
441322
Daucus carota
?
-
-
-
-
2.1.1.14
additional information
most active with the polyglutamate forms of N5-methyltetrahydropteroylglutamate
441322
Phaseolus sp.
?
-
-
-
-
2.1.1.14
additional information
final step in methionine synthesis
441322
Hordeum vulgare
?
-
-
-
-
2.1.1.14
additional information
final step in methionine synthesis
441322
Spinacia oleracea
?
-
-
-
-
2.1.1.14
additional information
final step in methionine synthesis
441322
Pisum sativum
?
-
-
-
-
2.1.1.14
additional information
final step in methionine synthesis
441322
Daucus carota
?
-
-
-
-
2.1.1.14
additional information
final step in methionine synthesis
441322
Phaseolus sp.
?
-
-
-
-
2.1.1.14
N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
441322
Pisum sativum
tetrahydropteroylmonoglutamate + L-methionine
-
-
-
-
2.1.1.14
S-adenosylmethionine + homocysteine
-
441322
Pisum sativum
methionine + S-adenosylhomocysteine
-
441322
Pisum sativum
?
2.1.1.14
S-methylmethionine + homocysteine
-
441322
Pisum sativum
methionine
-
441322
Pisum sativum
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.14
6.5
-
5-methyltetrahydropteroylglutamate
Pisum sativum
2.1.1.14
7.3
-
L-methionine
Pisum sativum
2.1.1.14
7.9
-
L-methionine
Phaseolus sp.
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.14
additional information
no requirement for a reducing system; no requirement for S-adenosylmethionine; vitamin-B12 independent enzyme
Daucus carota
2.1.1.14
additional information
no requirement for a reducing system; no requirement for S-adenosylmethionine; vitamin-B12 independent enzyme
Hordeum vulgare
2.1.1.14
additional information
no requirement for a reducing system; no requirement for S-adenosylmethionine; vitamin-B12 independent enzyme
Phaseolus sp.
2.1.1.14
additional information
no requirement for a reducing system; no requirement for S-adenosylmethionine; vitamin-B12 independent enzyme
Pisum sativum
2.1.1.14
additional information
no requirement for a reducing system; no requirement for S-adenosylmethionine; vitamin-B12 independent enzyme
Spinacia oleracea
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.14
additional information
no requirement for a reducing system; no requirement for S-adenosylmethionine; vitamin-B12 independent enzyme
Daucus carota
2.1.1.14
additional information
no requirement for a reducing system; no requirement for S-adenosylmethionine; vitamin-B12 independent enzyme
Hordeum vulgare
2.1.1.14
additional information
no requirement for a reducing system; no requirement for S-adenosylmethionine; vitamin-B12 independent enzyme
Phaseolus sp.
2.1.1.14
additional information
no requirement for a reducing system; no requirement for S-adenosylmethionine; vitamin-B12 independent enzyme
Pisum sativum
2.1.1.14
additional information
no requirement for a reducing system; no requirement for S-adenosylmethionine; vitamin-B12 independent enzyme
Spinacia oleracea
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.14
methionine
-
Daucus carota
2.1.1.14
methionine
-
Hordeum vulgare
2.1.1.14
methionine
-
Phaseolus sp.
2.1.1.14
methionine
-
Pisum sativum
2.1.1.14
methionine
-
Spinacia oleracea
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.14
0.000026
-
5-methyltetrahydropteroylglutamate
-
Pisum sativum
2.1.1.14
0.0022
-
S-methylmethionine
-
Pisum sativum
2.1.1.14
0.004
-
S-adenosylmethionine
-
Pisum sativum
2.1.1.14
0.041
-
homocysteine
cosubstrate S-methyl-L-methionine
Phaseolus sp.
2.1.1.14
0.055
-
S-methylmethionine
-
Phaseolus sp.
2.1.1.14
0.6
-
homocysteine
cosubstrate 5-methyltetrahydropteroylglutamate
Pisum sativum
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.1.14
chloroplast
-
Daucus carota
9507
-
2.1.1.14
chloroplast
-
Hordeum vulgare
9507
-
2.1.1.14
chloroplast
-
Phaseolus sp.
9507
-
2.1.1.14
chloroplast
-
Pisum sativum
9507
-
2.1.1.14
chloroplast
-
Spinacia oleracea
9507
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.1.1.14
Mg2+
stimulates
Daucus carota
2.1.1.14
Mg2+
stimulates
Hordeum vulgare
2.1.1.14
Mg2+
stimulates
Phaseolus sp.
2.1.1.14
Mg2+
stimulates
Pisum sativum
2.1.1.14
Mg2+
stimulates
Spinacia oleracea
2.1.1.14
phosphate
-
Daucus carota
2.1.1.14
phosphate
-
Hordeum vulgare
2.1.1.14
phosphate
-
Phaseolus sp.
2.1.1.14
phosphate
-
Pisum sativum
2.1.1.14
phosphate
-
Spinacia oleracea
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.14
additional information
Hordeum vulgare
final step in methionine synthesis
?
-
-
-
2.1.1.14
additional information
Spinacia oleracea
final step in methionine synthesis
?
-
-
-
2.1.1.14
additional information
Pisum sativum
final step in methionine synthesis
?
-
-
-
2.1.1.14
additional information
Daucus carota
final step in methionine synthesis
?
-
-
-
2.1.1.14
additional information
Phaseolus sp.
final step in methionine synthesis
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.14
-
Phaseolus sp.
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.14
root
-
Daucus carota
-
2.1.1.14
root
-
Hordeum vulgare
-
2.1.1.14
root
-
Phaseolus sp.
-
2.1.1.14
root
-
Pisum sativum
-
2.1.1.14
root
-
Spinacia oleracea
-
2.1.1.14
seedling
-
Daucus carota
-
2.1.1.14
seedling
-
Hordeum vulgare
-
2.1.1.14
seedling
-
Phaseolus sp.
-
2.1.1.14
seedling
-
Pisum sativum
-
2.1.1.14
seedling
-
Spinacia oleracea
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.14
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
441322
Hordeum vulgare
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
441322
Hordeum vulgare
?
2.1.1.14
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
441322
Spinacia oleracea
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
441322
Spinacia oleracea
?
2.1.1.14
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
441322
Pisum sativum
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
441322
Pisum sativum
?
2.1.1.14
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
441322
Daucus carota
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
441322
Daucus carota
?
2.1.1.14
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
441322
Phaseolus sp.
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
441322
Phaseolus sp.
?
2.1.1.14
additional information
enzymes of higher plants can use the monoglutamate
441322
Hordeum vulgare
?
-
-
-
-
2.1.1.14
additional information
enzymes of higher plants can use the monoglutamate
441322
Spinacia oleracea
?
-
-
-
-
2.1.1.14
additional information
enzymes of higher plants can use the monoglutamate
441322
Pisum sativum
?
-
-
-
-
2.1.1.14
additional information
enzymes of higher plants can use the monoglutamate
441322
Daucus carota
?
-
-
-
-
2.1.1.14
additional information
enzymes of higher plants can use the monoglutamate
441322
Phaseolus sp.
?
-
-
-
-
2.1.1.14
additional information
most active with the polyglutamate forms of N5-methyltetrahydropteroylglutamate
441322
Hordeum vulgare
?
-
-
-
-
2.1.1.14
additional information
most active with the polyglutamate forms of N5-methyltetrahydropteroylglutamate
441322
Spinacia oleracea
?
-
-
-
-
2.1.1.14
additional information
most active with the polyglutamate forms of N5-methyltetrahydropteroylglutamate
441322
Pisum sativum
?
-
-
-
-
2.1.1.14
additional information
most active with the polyglutamate forms of N5-methyltetrahydropteroylglutamate
441322
Daucus carota
?
-
-
-
-
2.1.1.14
additional information
most active with the polyglutamate forms of N5-methyltetrahydropteroylglutamate
441322
Phaseolus sp.
?
-
-
-
-
2.1.1.14
additional information
final step in methionine synthesis
441322
Hordeum vulgare
?
-
-
-
-
2.1.1.14
additional information
final step in methionine synthesis
441322
Spinacia oleracea
?
-
-
-
-
2.1.1.14
additional information
final step in methionine synthesis
441322
Pisum sativum
?
-
-
-
-
2.1.1.14
additional information
final step in methionine synthesis
441322
Daucus carota
?
-
-
-
-
2.1.1.14
additional information
final step in methionine synthesis
441322
Phaseolus sp.
?
-
-
-
-
2.1.1.14
N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
441322
Pisum sativum
tetrahydropteroylmonoglutamate + L-methionine
-
-
-
-
2.1.1.14
S-adenosylmethionine + homocysteine
-
441322
Pisum sativum
methionine + S-adenosylhomocysteine
-
441322
Pisum sativum
?
2.1.1.14
S-methylmethionine + homocysteine
-
441322
Pisum sativum
methionine
-
441322
Pisum sativum
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.14
6.5
-
5-methyltetrahydropteroylglutamate
Pisum sativum
2.1.1.14
7.3
-
L-methionine
Pisum sativum
2.1.1.14
7.9
-
L-methionine
Phaseolus sp.