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Literature summary extracted from

  • Pech, L.L.; Nelson, D.L.
    Purification and characterization of calmodulin (lysine 115) N-methyltransferase from Paramecium tetraurelia (1994), Biochim. Biophys. Acta, 1199, 183-194.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.60 dithiothreitol absolute requirement Paramecium tetraurelia

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.60 Calmidazolium
-
 Paramecium tetraurelia
2.1.1.60 Mellitin
-
 Paramecium tetraurelia
2.1.1.60 additional information not inhibitory: sinefungin, tubercidin, calmodulin antagonist W7 Paramecium tetraurelia
2.1.1.60 S-adenosylhomocysteine
-
 Paramecium tetraurelia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.60 0.001
-
 S-adenosyl-L-methionine
-
 Paramecium tetraurelia

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.60 Ca2+ stimulation Paramecium tetraurelia
2.1.1.60 Mg2+ activation Paramecium tetraurelia
2.1.1.60 Mn2+ stimulation Paramecium tetraurelia
2.1.1.60 additional information not stimulating: Zn2+ Paramecium tetraurelia
2.1.1.60 Ni2+ stimulation Paramecium tetraurelia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.60 33000
-
 gel filtration Paramecium tetraurelia
2.1.1.60 37000
-
 1 * 37000, SDS-PAGE Paramecium tetraurelia

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.60 Paramecium tetraurelia
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.60
-
 Paramecium tetraurelia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.60 S-adenosyl-L-methionine + calmodulin L-lysine
-
 Paramecium tetraurelia S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine trimethyllysine calmodulin, major product, + dimethyllysine + monomethyllysine ?

Subunits

EC Number Subunits Comment Organism
2.1.1.60 monomer 1 * 37000, SDS-PAGE Paramecium tetraurelia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.60 7.5
-
-
 Paramecium tetraurelia