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Literature summary extracted from

  • Tobin, M.B.; Cole, S.C.J.; Kovacevic, S.; Miller, J.R.; Baldwin, J.E.; Sutherland, J.D.
    Acyl-coenzyme A:isopenicillin N acyltransferase from Penicillium chrysogenum: effect of amino acid substitutions at Ser227, Ser230 and Ser309 on proenzyme cleavage and activity (1994), FEMS Microbiol. Lett., 121, 39-46.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.164 expressed in Escherichia coli JM109 Penicillium chrysogenum

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.164 S227A produces uncleaved proenzyme lacking enzyme activity Penicillium chrysogenum
2.3.1.164 S309A recombinant proenzyme containing S309A is cleaved, however enzyme activity is not observed Penicillium chrysogenum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.164 11000
-
 alpha 1 * 11000 + beta 1 * 29000, wild-type and recombinant enzyme, SDS-PAGE Penicillium chrysogenum
2.3.1.164 29000
-
 alpha 1 * 11000 + beta 1 * 29000, wild-type and recombinant enzyme, SDS-PAGE Penicillium chrysogenum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.164 phenylacetyl-CoA + isopenicillin N + H2O Penicillium chrysogenum
-
 CoA + benzylpenicillin + L-2-aminohexanedioate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.164 Penicillium chrysogenum
-
 SC6140, ATCC 2044, source of native enzyme
-
2.3.1.164 Penicillium chrysogenum
-
 penDE genes
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.164 phenylacetyl-CoA + 6-aminopenicillanic acid + H2O acyl-CoA:6-aminopenicillanate acyltransferase activity Penicillium chrysogenum CoA + benzylpenicillin
-
 ?
2.3.1.164 phenylacetyl-CoA + isopenicillin N + H2O
-
 Penicillium chrysogenum CoA + benzylpenicillin + L-2-aminohexanedioate
-
 ?
2.3.1.164 phenylacetyl-CoA + isopenicillin N + H2O acyl-coenzyme A:isopenicillin N acyltransferase activity, S309 is involved in substrate acylation Penicillium chrysogenum CoA + benzylpenicillin + L-2-aminohexanedioate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.164 heterodimer alpha 1 * 11000 + beta 1 * 29000, wild-type and recombinant enzyme, SDS-PAGE Penicillium chrysogenum