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Literature summary extracted from

  • Björk, G.R.; Svensson, I.
    Studies on microbial RNA. Fractionation of tRNA methylases from Saccharomyces cerevisiae (1969), Eur. J. Biochem., 9, 207-215.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.1.1.33 freezing without antifreeze agents inactivates, 30% ethylene glycol at -20°C stabilizes Saccharomyces cerevisiae
2.1.1.35 freezing without antifreeze agents inactivates, 30% ethylene glycol at -20°C stabilizes, dialysis inactivates, best pH used for dialysis is 6.5 Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.35 Mg2+ inhibits at low concentrations Saccharomyces cerevisiae
2.1.1.35 Mn2+ inhibits at low concentrations Saccharomyces cerevisiae
2.1.1.35 NH4+ at 0.1 M, 50% inhibition Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.35 additional information no divalent cations required, not activated by Mg2+ and Mn2+ Saccharomyces cerevisiae

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.1.1.33 Ethylene glycol 30% stabilizes at -20°C Saccharomyces cerevisiae
2.1.1.35 Ethylene glycol 30%, stabilizes at -20°C Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.33 Saccharomyces cerevisiae
-
-
-
2.1.1.33 Saccharomyces cerevisiae D84
-
-
-
2.1.1.35 Saccharomyces cerevisiae
-
-
-
2.1.1.35 Saccharomyces cerevisiae D84
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.33
-
 Saccharomyces cerevisiae
2.1.1.35 may be 3 isoenzymes Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.33 S-adenosyl-L-methionine + guanine46 in tRNA methyl group acceptor: methyl-deficient tRNA from E. coli Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
 ?
2.1.1.33 S-adenosyl-L-methionine + guanine46 in tRNA methyl group acceptor: methyl-deficient tRNA from E. coli Saccharomyces cerevisiae D84 S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
 ?
2.1.1.35 S-adenosyl-L-methionine + uridine54 in tRNA methyl group acceptor: methyl-deficient tRNA from E. coli Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
 ?
2.1.1.35 S-adenosyl-L-methionine + uridine54 in tRNA methyl group acceptor: methyl-deficient tRNA from E. coli Saccharomyces cerevisiae D84 S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.33 30
-
 assay at Saccharomyces cerevisiae
2.1.1.35 30
-
 assay at Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.33 S-adenosyl-L-methionine
-
 Saccharomyces cerevisiae
2.1.1.35 S-adenosyl-L-methionine
-
 Saccharomyces cerevisiae