BRENDA - Enzyme Database show

Substrate specificity of nicotinamide methyltransferase isolated from porcine liver

Alston, T.A.; Abeles, R.H.; Arch. Biochem. Biophys. 260, 601-608 (1988)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.1.1.1
glycerol stabilizes
Sus scrofa
2.1.1.1
thiols required for stability during isolation
Sus scrofa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.1
0.016
-
S-adenosyl-L-methionine
-
Sus scrofa
2.1.1.1
0.2
-
1,2,3,4-tetrahydroisoquinoline
-
Sus scrofa
2.1.1.1
0.83
-
nicotinamide
-
Sus scrofa
2.1.1.1
1.3
-
Quinoline
-
Sus scrofa
2.1.1.1
1.5
-
Isoquinoline
-
Sus scrofa
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.1
27000
30000
gel filtration, SDS-PAGE
Sus scrofa
2.1.1.1
27000
-
1 * 27000, SDS-PAGE
Sus scrofa
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.1
additional information
Sus scrofa
enzyme may function in detoxificating of numerous alkaloids
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.1
Sus scrofa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.1.1
-
Sus scrofa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.1
liver
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.1.1
additional information
-
assay
Sus scrofa
Storage Stability
EC Number
Storage Stability
Organism
2.1.1.1
-20°C, 20% glycerol w/v, stable for 1 month, partially purified enzyme
Sus scrofa
2.1.1.1
4°C, 20% glycerol w/v, pH 7.6, 30% loss of activity, purified enzyme
Sus scrofa
2.1.1.1
4°C, 20% w/v glycerol, stable for 1 week, partially purified enzyme
Sus scrofa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.1
additional information
enzyme may function in detoxificating of numerous alkaloids
441252
Sus scrofa
?
-
-
-
-
2.1.1.1
S-adenosyl-L-methionine + 1,2,3,4-tetrahydroisoquinoline
-
441252
Sus scrofa
S-adenosyl-L-homocysteine + ?
-
-
-
?
2.1.1.1
S-adenosyl-L-methionine + 3-acetylpyridine
-
441252
Sus scrofa
S-adenosyl-L-homocysteine + ?
-
-
-
?
2.1.1.1
S-adenosyl-L-methionine + isoquinoline
-
441252
Sus scrofa
S-adenosyl-L-homocysteine + ?
-
-
-
?
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
-
441252
Sus scrofa
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
-
?
2.1.1.1
S-adenosyl-L-methionine + quinoline
-
441252
Sus scrofa
S-adenosyl-L-homocysteine + ?
-
-
-
?
2.1.1.1
S-adenosyl-L-methionine + thionicotinamide
-
441252
Sus scrofa
S-adenosyl-L-homocysteine + 3-carbamothioyl-1-methylpyridinium
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.1.1.1
monomer
1 * 27000, SDS-PAGE
Sus scrofa
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.1.1.1
25
-
assay at
Sus scrofa
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.1
7.2
-
-
Sus scrofa
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.1.1.1
5.7
9.8
about 50% of maximum activity at pH 5.7 and 9.8
Sus scrofa
General Stability (protein specific)
EC Number
General Stability
Organism
2.1.1.1
glycerol stabilizes
Sus scrofa
2.1.1.1
thiols required for stability during isolation
Sus scrofa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.1
0.016
-
S-adenosyl-L-methionine
-
Sus scrofa
2.1.1.1
0.2
-
1,2,3,4-tetrahydroisoquinoline
-
Sus scrofa
2.1.1.1
0.83
-
nicotinamide
-
Sus scrofa
2.1.1.1
1.3
-
Quinoline
-
Sus scrofa
2.1.1.1
1.5
-
Isoquinoline
-
Sus scrofa
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.1
27000
30000
gel filtration, SDS-PAGE
Sus scrofa
2.1.1.1
27000
-
1 * 27000, SDS-PAGE
Sus scrofa
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.1
additional information
Sus scrofa
enzyme may function in detoxificating of numerous alkaloids
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.1
-
Sus scrofa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.1
liver
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.1.1
additional information
-
assay
Sus scrofa
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.1.1.1
-20°C, 20% glycerol w/v, stable for 1 month, partially purified enzyme
Sus scrofa
2.1.1.1
4°C, 20% glycerol w/v, pH 7.6, 30% loss of activity, purified enzyme
Sus scrofa
2.1.1.1
4°C, 20% w/v glycerol, stable for 1 week, partially purified enzyme
Sus scrofa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.1
additional information
enzyme may function in detoxificating of numerous alkaloids
441252
Sus scrofa
?
-
-
-
-
2.1.1.1
S-adenosyl-L-methionine + 1,2,3,4-tetrahydroisoquinoline
-
441252
Sus scrofa
S-adenosyl-L-homocysteine + ?
-
-
-
?
2.1.1.1
S-adenosyl-L-methionine + 3-acetylpyridine
-
441252
Sus scrofa
S-adenosyl-L-homocysteine + ?
-
-
-
?
2.1.1.1
S-adenosyl-L-methionine + isoquinoline
-
441252
Sus scrofa
S-adenosyl-L-homocysteine + ?
-
-
-
?
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
-
441252
Sus scrofa
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
-
?
2.1.1.1
S-adenosyl-L-methionine + quinoline
-
441252
Sus scrofa
S-adenosyl-L-homocysteine + ?
-
-
-
?
2.1.1.1
S-adenosyl-L-methionine + thionicotinamide
-
441252
Sus scrofa
S-adenosyl-L-homocysteine + 3-carbamothioyl-1-methylpyridinium
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.1.1.1
monomer
1 * 27000, SDS-PAGE
Sus scrofa
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.1.1.1
25
-
assay at
Sus scrofa
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.1
7.2
-
-
Sus scrofa
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.1.1.1
5.7
9.8
about 50% of maximum activity at pH 5.7 and 9.8
Sus scrofa