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Literature summary extracted from

  • Finkelstein, J.D.; Harris, B.J.; Kyle, W.E.
    Methionine metabolism in mammals: kinetic study of betaine-homocysteine methyltransferase (1972), Arch. Biochem. Biophys., 153, 320-324.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.5 D-cysteine increase of product formation Rattus norvegicus
2.1.1.5 dithiothreitol
-
 Rattus norvegicus
2.1.1.5 L-cysteine stimulation at low concentration Rattus norvegicus
2.1.1.5 additional information taurine, cysteic acid, cysteine sulfinate, sulfate, glycine, L-serine, L-threonine do not affect activity Rattus norvegicus
2.1.1.5 S-[(2R)-2-amino-2-carboxyethyl]-L-homocysteine
-
 Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.5 D-Cystine
-
 Rattus norvegicus
2.1.1.5 L-cysteine
-
 Rattus norvegicus
2.1.1.5 L-cystine
-
 Rattus norvegicus
2.1.1.5 additional information not inhibitory: taurine, cysteic acid, cysteine sulfinate, sulfate, glycine, L-serine, L-threonine; product inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.5 0.012 0.021 L-homocysteine
-
 Rattus norvegicus
2.1.1.5 0.039 0.056 betaine
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.5 N,N,N-trimethylglycine + L-homocysteine Rattus norvegicus i.e. betaine, involved in betaine metabolism N,N-dimethylglycine + L-methionine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.5 Rattus norvegicus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.5 betaine + L-homocysteine = dimethylglycine + L-methionine ordered bi-bi mechanism with homocysteine the first substrate to be added and methionine the last product released Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.5 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.5 N,N,N-trimethylglycine + L-homocysteine
-
 Rattus norvegicus N,N-dimethylglycine + L-methionine
-
 ?
2.1.1.5 N,N,N-trimethylglycine + L-homocysteine i.e. betaine, involved in betaine metabolism Rattus norvegicus N,N-dimethylglycine + L-methionine
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.5 additional information
-
 Tris, TES and HEPES decrease activity Rattus norvegicus
2.1.1.5 8
-
 0.05 M Tricine buffer Rattus norvegicus