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Literature summary extracted from

  • Grabau, W.; Rudiger, H.
    Purification and properties of the tetrahydropteroylglutamate methyltransferase from green beans (Phaseolus vulgaris) (1977), Hoppe-Seyler's Z. Physiol. Chem., 358, 695-698.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.13 0.43
-
 L-homocysteine
-
 Phaseolus vulgaris
2.1.1.13 2.4
-
 N5-methyltetrahydropteroylmonoglutamate
-
 Phaseolus vulgaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.13 Mg2+ activates Phaseolus vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.13 40000
-
 gel filtration Phaseolus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.13 Phaseolus vulgaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.13
-
 Phaseolus vulgaris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.13 additional information
-
 assay method Phaseolus vulgaris

Storage Stability

EC Number Storage Stability Organism
2.1.1.13 0°C, pH 7, half-life 6 days Phaseolus vulgaris
2.1.1.13 freeze-dried, 20% loss of activity after 3 months Phaseolus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.13 N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
 Phaseolus vulgaris tetrahydropteroylmonoglutamate + L-methionine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.13 31
-
-
 Phaseolus vulgaris
2.1.1.13 39
-
-
 Phaseolus vulgaris

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.1.1.13 10 39 10°C: about 10% of maximum activity, 39°C: maximum activity Phaseolus vulgaris
2.1.1.13 15 31 15°C: about 10% of maximum activity, 31°C: maximum activity Phaseolus vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.13 6.5
-
-
 Phaseolus vulgaris

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.1.1.13 7
-
 stability optimum, 0°C, t1/2: 6 days Phaseolus vulgaris