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Literature summary extracted from

  • Loughlin, R.E.; Elford, H.L.; Buchanan, J.M.
    Enzymatic synthesis of the methyl group of methionine (1964), J. Biol. Chem., 239, 2888-2895.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.1.1.13 acetone inactivates Sus scrofa
2.1.1.13 freezing and thawing, 40% loss of activity Sus scrofa
2.1.1.13 mercaptoethanol, 0.2 M, inactivation after 2 h at 24°C or during freezing and thawing, no effect at 0°C Sus scrofa
2.1.1.13 precipitation at pH 5 inactivates Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.13 5-methyltetrahydrofolate + L-homocysteine Sus scrofa methionine biosynthesis tetrahydrofolate + L-methionine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.13 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.13
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.13 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.13 additional information
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.1.1.13 0°C, glutathione, N2-atmosphere, stable for at least a week Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.13 5-methyltetrahydrofolate + L-homocysteine methionine biosynthesis Sus scrofa tetrahydrofolate + L-methionine
-
 ?
2.1.1.13 N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
 Sus scrofa tetrahydropteroylmonoglutamate + L-methionine
-
 ?
2.1.1.13 N5-methyltetrahydropteroyltriglutamate + L-homocysteine
-
 Sus scrofa tetrahydropteroyltriglutamate + L-methionine
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.13 37
-
 2 h, complete loss of activity Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.13 7
-
-
 Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.13 6.2 8.2 pH about 50% of maximum activity at pH 6.2 and 8.2 Sus scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.1.1.13 5
-
 precipitation at pH 5 inactivates Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.13 FAD enzyme requires reduced FAD and S-adenosyl-L-methionine Sus scrofa
2.1.1.13 S-adenosyl-L-methionine bacterial enzyme requires reduced FAD and S-adenosyl-L-methionine Sus scrofa
2.1.1.13 vitamin B12 enzyme contains a derivative of vitamin B12 as prosthetic group Sus scrofa