BRENDA - Enzyme Database

Transfer of the methyl group from N5-methyltetrahydrofolates to homocysteine in Escherichia coli

Guest, J.R.; Friedman, S.; Foster, M.A.; Tejerina, G.; Woods, D.D.; Biochem. J. 92, 497-504 (1964)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.14
Mn2+
above 1 mM
Escherichia coli
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.1.1.13
Mg2+
required
Escherichia coli
2.1.1.14
Mg2+
required
Escherichia coli
2.1.1.14
Mn2+
below 1 mM more effective than Mg2+, above 1 mM inhibitory
Escherichia coli
2.1.1.14
phosphate
-
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.13
Escherichia coli
-
B
-
2.1.1.13
Escherichia coli B / ATCC 11303
-
B
-
2.1.1.14
Escherichia coli
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.13
additional information
S-adenosylhomocysteine replaces homocysteine as methyl acceptor to a limited extent
441172
Escherichia coli
?
-
-
-
-
2.1.1.13
additional information
S-adenosylhomocysteine replaces homocysteine as methyl acceptor to a limited extent
441172
Escherichia coli B / ATCC 11303
?
-
-
-
-
2.1.1.13
N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
441172
Escherichia coli
tetrahydropteroylmonoglutamate + L-methionine
-
-
-
?
2.1.1.13
N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
441172
Escherichia coli B / ATCC 11303
tetrahydropteroylmonoglutamate + L-methionine
-
-
-
?
2.1.1.13
N5-methyltetrahydropteroyltriglutamate + L-homocysteine
-
441172
Escherichia coli
tetrahydropteroyltriglutamate + L-methionine
-
-
-
?
2.1.1.13
N5-methyltetrahydropteroyltriglutamate + L-homocysteine
-
441172
Escherichia coli B / ATCC 11303
tetrahydropteroyltriglutamate + L-methionine
-
-
-
?
2.1.1.14
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
441172
Escherichia coli
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
-
-
?
2.1.1.14
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
441172
Escherichia coli B / ATCC 11303
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
-
-
?
2.1.1.14
additional information
monoglutamate analog not used
441172
Escherichia coli
?
-
-
-
-
2.1.1.14
additional information
monoglutamate analog not used
441172
Escherichia coli B / ATCC 11303
?
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.14
8.1
-
-
Escherichia coli
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.1.1.14
6
8.7
pH 6.0: about 70% of maximum activity, pH 8.7: about 90% of maximum activity
Escherichia coli
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.13
ATP
required
Escherichia coli
2.1.1.13
additional information
enzyme requires another protein fraction, ATP, Mg2+, FAD and a NADH-generating system
Escherichia coli
2.1.1.13
vitamin B12
enzyme contains a derivative of vitamin B12 as prosthetic group
Escherichia coli
2.1.1.14
additional information
vitamin-B12 independent enzyme
Escherichia coli
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.13
ATP
required
Escherichia coli
2.1.1.13
additional information
enzyme requires another protein fraction, ATP, Mg2+, FAD and a NADH-generating system
Escherichia coli
2.1.1.13
vitamin B12
enzyme contains a derivative of vitamin B12 as prosthetic group
Escherichia coli
2.1.1.14
additional information
vitamin-B12 independent enzyme
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.14
Mn2+
above 1 mM
Escherichia coli
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.1.1.13
Mg2+
required
Escherichia coli
2.1.1.14
Mg2+
required
Escherichia coli
2.1.1.14
Mn2+
below 1 mM more effective than Mg2+, above 1 mM inhibitory
Escherichia coli
2.1.1.14
phosphate
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.13
additional information
S-adenosylhomocysteine replaces homocysteine as methyl acceptor to a limited extent
441172
Escherichia coli
?
-
-
-
-
2.1.1.13
additional information
S-adenosylhomocysteine replaces homocysteine as methyl acceptor to a limited extent
441172
Escherichia coli B / ATCC 11303
?
-
-
-
-
2.1.1.13
N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
441172
Escherichia coli
tetrahydropteroylmonoglutamate + L-methionine
-
-
-
?
2.1.1.13
N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
441172
Escherichia coli B / ATCC 11303
tetrahydropteroylmonoglutamate + L-methionine
-
-
-
?
2.1.1.13
N5-methyltetrahydropteroyltriglutamate + L-homocysteine
-
441172
Escherichia coli
tetrahydropteroyltriglutamate + L-methionine
-
-
-
?
2.1.1.13
N5-methyltetrahydropteroyltriglutamate + L-homocysteine
-
441172
Escherichia coli B / ATCC 11303
tetrahydropteroyltriglutamate + L-methionine
-
-
-
?
2.1.1.14
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
441172
Escherichia coli
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
-
-
?
2.1.1.14
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
441172
Escherichia coli B / ATCC 11303
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
-
-
?
2.1.1.14
additional information
monoglutamate analog not used
441172
Escherichia coli
?
-
-
-
-
2.1.1.14
additional information
monoglutamate analog not used
441172
Escherichia coli B / ATCC 11303
?
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.14
8.1
-
-
Escherichia coli
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.1.1.14
6
8.7
pH 6.0: about 70% of maximum activity, pH 8.7: about 90% of maximum activity
Escherichia coli