BRENDA - Enzyme Database show

A cyanobacterial narB gene encodes a ferredoxin-dependent nitrate reductase

Rubio, L.M.; Herrero, A.; Flores, E.; Plant Mol. Biol. 30, 845-850 (1996)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.7.7.2
narB structural gene for nitrate reductase is cloned and expressed in Escherichia coli as a 76 kDa polypeptide with 729 amino acids
Synechococcus sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.7.7.2
0.7
2.1
nitrate
-
Synechococcus sp.
1.7.7.2
1.5
-
nitrate
reduced methyl viologen as electron donor, recombinant enzyme expressed in Escherichia coli
Synechococcus sp.
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.7.7.2
soluble
recombinant enzyme activity is found exclusively in the soluble fraction
Synechococcus sp.
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.7.7.2
76000
-
x * 76000, enzyme expressed in Escherichia coli, SDS-PAGE
Synechococcus sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.7.7.2
nitrate + reduced ferredoxin
Synechococcus sp.
assimilatory nitrate reduction
nitrite + oxidized ferredoxin
-
Synechococcus sp.
?
1.7.7.2
nitrate + reduced ferredoxin
Synechococcus sp.
reduced ferredoxin as physiological electron donor
nitrite + oxidized ferredoxin
-
Synechococcus sp.
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.7.7.2
Synechococcus sp.
-
strain PCC 7942
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.7.7.2
nitrate + reduced ferredoxin
ratio of methyl viologen- to ferredoxin-dependent nitrate reduction is about 27
440454
Synechococcus sp.
nitrite + H2O + oxidized ferredoxin
-
440454
Synechococcus sp.
?
1.7.7.2
nitrate + reduced ferredoxin
assimilatory nitrate reduction
440454
Synechococcus sp.
nitrite + oxidized ferredoxin
-
440454
Synechococcus sp.
?
1.7.7.2
nitrate + reduced ferredoxin
reduced ferredoxin as physiological electron donor
440454
Synechococcus sp.
nitrite + oxidized ferredoxin
-
440454
Synechococcus sp.
?
1.7.7.2
nitrate + reduced methyl viologen
enzyme uses also reduced methyl viologen as reductant
440454
Synechococcus sp.
nitrite + oxidized methyl viologen
-
440454
Synechococcus sp.
?
Subunits
EC Number
Subunits
Commentary
Organism
1.7.7.2
?
x * 76000, enzyme expressed in Escherichia coli, SDS-PAGE
Synechococcus sp.
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.7.7.2
37
-
reduced methyl viologen as electron donor
Synechococcus sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.7.7.2
9.5
10.5
reduced methyl viologen as electron donor
Synechococcus sp.
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.7.7.2
additional information
narB gene with domains that might be involved in binding of the molybdenum cofactor and of an iron-sulfur cluster, but no flavin nucleotide- or heme-binding domains
Synechococcus sp.
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.7.7.2
narB structural gene for nitrate reductase is cloned and expressed in Escherichia coli as a 76 kDa polypeptide with 729 amino acids
Synechococcus sp.
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.7.7.2
additional information
narB gene with domains that might be involved in binding of the molybdenum cofactor and of an iron-sulfur cluster, but no flavin nucleotide- or heme-binding domains
Synechococcus sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.7.7.2
0.7
2.1
nitrate
-
Synechococcus sp.
1.7.7.2
1.5
-
nitrate
reduced methyl viologen as electron donor, recombinant enzyme expressed in Escherichia coli
Synechococcus sp.
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.7.7.2
soluble
recombinant enzyme activity is found exclusively in the soluble fraction
Synechococcus sp.
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.7.7.2
76000
-
x * 76000, enzyme expressed in Escherichia coli, SDS-PAGE
Synechococcus sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.7.7.2
nitrate + reduced ferredoxin
Synechococcus sp.
assimilatory nitrate reduction
nitrite + oxidized ferredoxin
-
Synechococcus sp.
?
1.7.7.2
nitrate + reduced ferredoxin
Synechococcus sp.
reduced ferredoxin as physiological electron donor
nitrite + oxidized ferredoxin
-
Synechococcus sp.
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.7.7.2
nitrate + reduced ferredoxin
ratio of methyl viologen- to ferredoxin-dependent nitrate reduction is about 27
440454
Synechococcus sp.
nitrite + H2O + oxidized ferredoxin
-
440454
Synechococcus sp.
?
1.7.7.2
nitrate + reduced ferredoxin
assimilatory nitrate reduction
440454
Synechococcus sp.
nitrite + oxidized ferredoxin
-
440454
Synechococcus sp.
?
1.7.7.2
nitrate + reduced ferredoxin
reduced ferredoxin as physiological electron donor
440454
Synechococcus sp.
nitrite + oxidized ferredoxin
-
440454
Synechococcus sp.
?
1.7.7.2
nitrate + reduced methyl viologen
enzyme uses also reduced methyl viologen as reductant
440454
Synechococcus sp.
nitrite + oxidized methyl viologen
-
440454
Synechococcus sp.
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.7.7.2
?
x * 76000, enzyme expressed in Escherichia coli, SDS-PAGE
Synechococcus sp.
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.7.7.2
37
-
reduced methyl viologen as electron donor
Synechococcus sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.7.7.2
9.5
10.5
reduced methyl viologen as electron donor
Synechococcus sp.