EC Number | Cloned (Comment) | Organism |
---|---|---|
1.7.7.2 | narB structural gene for nitrate reductase is cloned and expressed in Escherichia coli as a 76 kDa polypeptide with 729 amino acids | Synechococcus sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.7.7.2 | 0.7 | 2.1 | nitrate | - |
Synechococcus sp. | |
1.7.7.2 | 1.5 | - |
nitrate | reduced methyl viologen as electron donor, recombinant enzyme expressed in Escherichia coli | Synechococcus sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.7.7.2 | soluble | recombinant enzyme activity is found exclusively in the soluble fraction | Synechococcus sp. | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.7.7.2 | 76000 | - |
x * 76000, enzyme expressed in Escherichia coli, SDS-PAGE | Synechococcus sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.7.7.2 | nitrate + reduced ferredoxin | Synechococcus sp. | assimilatory nitrate reduction | nitrite + oxidized ferredoxin | - |
? | |
1.7.7.2 | nitrate + reduced ferredoxin | Synechococcus sp. | reduced ferredoxin as physiological electron donor | nitrite + oxidized ferredoxin | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.7.7.2 | Synechococcus sp. | - |
strain PCC 7942 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.7.7.2 | nitrate + reduced ferredoxin | ratio of methyl viologen- to ferredoxin-dependent nitrate reduction is about 27 | Synechococcus sp. | nitrite + H2O + oxidized ferredoxin | - |
? | |
1.7.7.2 | nitrate + reduced ferredoxin | assimilatory nitrate reduction | Synechococcus sp. | nitrite + oxidized ferredoxin | - |
? | |
1.7.7.2 | nitrate + reduced ferredoxin | reduced ferredoxin as physiological electron donor | Synechococcus sp. | nitrite + oxidized ferredoxin | - |
? | |
1.7.7.2 | nitrate + reduced methyl viologen | enzyme uses also reduced methyl viologen as reductant | Synechococcus sp. | nitrite + oxidized methyl viologen | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.7.7.2 | ? | x * 76000, enzyme expressed in Escherichia coli, SDS-PAGE | Synechococcus sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.7.7.2 | 37 | - |
reduced methyl viologen as electron donor | Synechococcus sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.7.7.2 | 9.5 | 10.5 | reduced methyl viologen as electron donor | Synechococcus sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.7.7.2 | additional information | narB gene with domains that might be involved in binding of the molybdenum cofactor and of an iron-sulfur cluster, but no flavin nucleotide- or heme-binding domains | Synechococcus sp. |