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Literature summary extracted from

  • Mazzafera, P.; Robinson, S.P.
    Characterization of polyphenol oxidase in coffee (2000), Phytochemistry, 55, 285-296.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.1 Cinnamic acid
-
 Coffea arabica
1.10.3.1 hexadecyltrimethyl-ammonium bromide
-
 Coffea arabica
1.10.3.1 polyvinylpyrrolidone 40
-
 Coffea arabica
1.10.3.1 Salicylhydroxamic acid
-
 Coffea arabica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.1 0.88
-
 chlorogenic acid leaf enzyme Coffea arabica
1.10.3.1 2.27
-
 chlorogenic acid endosperm enzyme Coffea arabica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.10.3.1 chlorogenic acid + O2 Coffea arabica probably the major substrate in vivo ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.1 Coffea arabica
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.1 fruit endosperm Coffea arabica
-
1.10.3.1 leaf
-
 Coffea arabica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.1 4-methylcatechol + O2
-
 Coffea arabica 4-methyl-1,2-benzoquinone
-
 ?
1.10.3.1 chlorogenic acid + O2 best substrate for both leaf and endosperm enzyme Coffea arabica ?
-
 ?
1.10.3.1 chlorogenic acid + O2 probably the major substrate in vivo Coffea arabica ?
-
 ?
1.10.3.1 L-dopa + 1/2 O2 endosperm enzyme, 3.9% of activity with chlorogenic acid Coffea arabica L-dopaquinone + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.1 More enzyme may be synthesized as a 67000 Da precursor that can be hydrolyzed to a 45000 Da form still retaining catalytic activity Coffea arabica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.1 25 30 leaf and endosperm enzyme Coffea arabica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.1 50
-
 leaf enzyme, no loss of activity after 30 min, 50% loss of activity after 5 min at 80°C, endosperm enzyme, 20% loss of activity after 30 min at 50°C, 50% loss of activity after 2.5 min at 80°C Coffea arabica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.1 6 7 leaf and endosperm enzyme Coffea arabica