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Literature summary extracted from

  • Yang, C.P.; Fujita, S.; Ashrafuzzaman, M.; Nakamura, N.; Hayashi, N.
    Purification and characterization of polyphenol oxidase from banana (Musa sapientum L.) pulp (2000), J. Agric. Food Chem., 48, 2732-2735.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.1 ascorbic acid pulp enzyme, 1 mM, complete inhibition Musa x paradisiaca
1.10.3.1 CN- pulp enzyme, 1 mM, 80% inhibition Musa x paradisiaca
1.10.3.1 cysteine pulp enzyme, 1 mM, complete inhibition Musa x paradisiaca
1.10.3.1 diethyldithiocarbamate pulp enzyme, 1 mM, complete inhibition Musa x paradisiaca
1.10.3.1 additional information not inhibited by 10 mM EDTA, Cu2+, Mn2+, Zn2+, and Ba2+ Musa x paradisiaca

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.1 2.8
-
 dopamine
-
 Musa x paradisiaca

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.1 41000
-
 pulp enzyme, gel filtration Musa x paradisiaca
1.10.3.1 42000
-
 1 * 42000, SDS-PAGE Musa x paradisiaca

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.1 Musa x paradisiaca
-
 banana
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.1 ammonium sulfate, DEAE-toyopearl, butyl-toyopearl, Super Q toyopearl, hydroxyapatite, toyopearl HW 55 Musa x paradisiaca

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.1 pulp
-
 Musa x paradisiaca
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.1 additional information
-
 13160.0 units/mg, 1 unit is defined as absorbance change at 420 nm of 0.1 per min Musa x paradisiaca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.1 caffeic acid + 1/2 O2 2% of activity with dopamine Musa x paradisiaca caffeoyl quinone + H2O
-
 ?
1.10.3.1 catechol + 1/2 O2 pulp enzyme, 54% of activity with dopamine Musa x paradisiaca 1,2-benzoquinone + H2O
-
 ?
1.10.3.1 chlorogenic acid + O2 pulp enzyme, 24.5% of activity with dopamine Musa x paradisiaca ?
-
 ?
1.10.3.1 D-catechin + O2 pulp enzyme, 35.6% of activity with dopamine Musa x paradisiaca ?
-
 ?
1.10.3.1 dopamine + O2
-
 Musa x paradisiaca 4-(2-aminoethyl)-1,2-benzoquinone + 2 H2O
-
 ?
1.10.3.1 epicatechin + O2 pulp enzyme, 22.7% of activity with dopamine Musa x paradisiaca ?
-
 ?
1.10.3.1 L-dopa + 1/2 O2 pulp enzyme, 12.3% of activity with dopamine Musa x paradisiaca L-dopaquinone + H2O
-
 ?
1.10.3.1 pyrogallol + O2 pulp enzyme, 5.5% of activity with dopamine Musa x paradisiaca ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.1 monomer 1 * 42000, SDS-PAGE Musa x paradisiaca

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.1 30
-
-
 Musa x paradisiaca

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.1 70
-
 pulp enzyme, loss of 20% activity after 10 min Musa x paradisiaca

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.1 6.5
-
-
 Musa x paradisiaca

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.1 5 11 10% loss of activity after 48 h between pH 5.0 and 11.0 Musa x paradisiaca