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Literature summary extracted from
- Lactate oxygenase of Mycobacterium phlei (1975), Methods Enzymol., 41B, 329-333.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.12.4 | (R)-lactate | competitive inhibitor | Mycolicibacterium phlei |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.12.4 | 34 | - |
(S)-lactate | - |
Mycolicibacterium phlei | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.13.12.4 | 56000 | - |
6 * 56000, flavin and terminal amino acid analysis | Mycolicibacterium phlei |
| 1.13.12.4 | 58600 | - |
6 * 58600, electron microscopic analysis | Mycolicibacterium phlei |
| 1.13.12.4 | 340000 | - |
gel filtration | Mycolicibacterium phlei |
| 1.13.12.4 | 352000 | - |
low speed sedimentation | Mycolicibacterium phlei |
| 1.13.12.4 | 370000 | - |
sedimentation equilibrium analysis | Mycolicibacterium phlei |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.12.4 | (S)-lactate + O2 | Mycolicibacterium phlei | - |
acetate + CO2 + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.12.4 | Mycolicibacterium phlei | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.13.12.4 | - |
Mycolicibacterium phlei |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.13.12.4 | 32 | - |
- |
Mycolicibacterium phlei |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.13.12.4 | 5°C, crystalline suspension in ammonium sulfate, several months, no loss of activity | Mycolicibacterium phlei |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.12.4 | (S)-lactate + 2,6-dichlorophenolindophenol | no decarboxylation | Mycolicibacterium phlei | pyruvate + ? | - |
? | |
| 1.13.12.4 | (S)-lactate + O2 | - |
Mycolicibacterium phlei | acetate + CO2 + H2O | - |
? | |
| 1.13.12.4 | (S)-malate + O2 | - |
Mycolicibacterium phlei | oxaloacetate + H2O2 | - |
? | |
| 1.13.12.4 | 2-hydroxybutyrate + O2 | - |
Mycolicibacterium phlei | ? | - |
? | |
| 1.13.12.4 | 2-hydroxycaproate + O2 | - |
Mycolicibacterium phlei | ? | - |
? | |
| 1.13.12.4 | 2-hydroxyvalerate + O2 | - |
Mycolicibacterium phlei | ? | - |
? | |
| 1.13.12.4 | beta-phenyllactate + O2 | - |
Mycolicibacterium phlei | ? | - |
? | |
| 1.13.12.4 | DL-mandelate + O2 | - |
Mycolicibacterium phlei | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.13.12.4 | hexamer | 6 * 58600, electron microscopic analysis | Mycolicibacterium phlei |
| 1.13.12.4 | hexamer | 6 * 56000, flavin and terminal amino acid analysis | Mycolicibacterium phlei |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.12.4 | 6 | - |
- |
Mycolicibacterium phlei |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.12.4 | FMN | - |
Mycolicibacterium phlei | |
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