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Literature summary extracted from

  • Muh, U.; Massey, V.; Williams, C.H., Jr.
    Lactate Monooxygenase. I. Expression of the mycobacterial gene in Escherichia coli and site-directed mutagenesis of lysine 266 (1994), J. Biol. Chem., 269, 7982-7988.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.4 expressed in Escherichia coli BL21 Mycolicibacterium smegmatis

Protein Variants

EC Number Protein Variants Comment Organism
1.13.12.4 K266M the rate of reduction with (S)-lactate is decreased compared with that of the wild-type enzyme, the mutant enzyme is virtually inactive Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.12.4 (S)-lactate + O2 Mycolicibacterium smegmatis
-
 acetate + CO2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.4 Mycolicibacterium smegmatis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.4
-
 Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.4 (S)-lactate + O2
-
 Mycolicibacterium smegmatis acetate + CO2 + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.12.4 FMN
-
 Mycolicibacterium smegmatis