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Literature summary extracted from

  • Cortes, J.; Martin, J.F.; Castro, J.M.; Laiz, L.; Liras, P.
    Purification and characterization of a 2-oxoglutarate-linked ATP-independent deacetoxycephalosporin C synthase of Streptomyces lactamdurans (1987), J. Gen. Microbiol., 133, 3165-3174.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.14.20.1 increasing ionic strength to 100 decreases stability of the enzyme Amycolatopsis lactamdurans
1.14.20.1 the enzyme is very unstable and can be partially stabilized in 25 mM Tris-HCl, pH 9.0, in the presence of 0.1 mM DTT Amycolatopsis lactamdurans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.20.1 2-oxobutyrate 0.1 mM, complete inhibition Amycolatopsis lactamdurans
1.14.20.1 3-oxoadipate 0.1 mM, 56% inhibition Amycolatopsis lactamdurans
1.14.20.1 Co2+ 1 mM, 79% inhibition Amycolatopsis lactamdurans
1.14.20.1 Cu2+ 1 mM, complete inhibition Amycolatopsis lactamdurans
1.14.20.1 phosphate
-
 Amycolatopsis lactamdurans
1.14.20.1 Zn2+ 1 mM, 84% inhibition Amycolatopsis lactamdurans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.20.1 0.003
-
 oxoglutarate
-
 Amycolatopsis lactamdurans
1.14.20.1 0.052
-
 Penicillin N
-
 Amycolatopsis lactamdurans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.20.1 Fe2+ required Amycolatopsis lactamdurans
1.14.20.1 Fe2+ Km: 0.071 mM Amycolatopsis lactamdurans
1.14.20.1 Fe3+ can replace Fe2+ under reducing conditions Amycolatopsis lactamdurans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.20.1 27000
-
 gel filtration Amycolatopsis lactamdurans
1.14.20.1 28000
-
 1 * 28000, SDS-PAGE Amycolatopsis lactamdurans

Organism

EC Number Organism UniProt Comment Textmining
1.14.20.1 Amycolatopsis lactamdurans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.20.1
-
 Amycolatopsis lactamdurans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.20.1 additional information
-
-
 Amycolatopsis lactamdurans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.20.1 additional information no activity with isopenicillin N or 6-aminopenicillanic acid Amycolatopsis lactamdurans ?
-
 ?
1.14.20.1 penicillin G + 2-oxoglutarate + O2 no activity Amycolatopsis lactamdurans phenylacetyl-7-aminodeacetoxy cephalosporanic acid + succinate + CO2 + H2O
-
 ?
1.14.20.1 penicillin N + 2-oxoglutarate + O2 oxoglutarate analogs are not used as substrate Amycolatopsis lactamdurans deacetoxycephalosporin C + succinate + CO2 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.20.1 monomer 1 * 28000, SDS-PAGE Amycolatopsis lactamdurans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.20.1 25 30
-
 Amycolatopsis lactamdurans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.14.20.1 5 40 20°C: 15% of maximal activity, 35°C: 15% of maximal activity Amycolatopsis lactamdurans
1.14.20.1 20 35 20°C: 90% of maximal activity, 35°C: 90% of maximal activity Amycolatopsis lactamdurans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.20.1 25
-
 60 min, stable Amycolatopsis lactamdurans
1.14.20.1 30
-
 60 min, 25% loss of activity Amycolatopsis lactamdurans
1.14.20.1 65
-
 60 min, complete inactivation Amycolatopsis lactamdurans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.20.1 5 11
-
 Amycolatopsis lactamdurans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.20.1 7
-
 4°C, 120 h, 80% loss of activity Amycolatopsis lactamdurans
1.14.20.1 8
-
 4°C, 120 h, 70% loss of activity Amycolatopsis lactamdurans
1.14.20.1 9
-
 4°C, 120 h, 35% loss of activity Amycolatopsis lactamdurans