Literature summary extracted from

Lloyd, M.D.; Lee, H.J.; Harlos, K.; Zhang, Z.H.; Baldwin, J.E.; Schofield, C.J.; Charnock, J.M.; Garner, C.D.; Hara, T.; Terwisscha van Scheltinga, A.C.; Valeg.Ang.rd, K.; Viklund, J.A.C.; Hajdu, J.; Andersson, I.; Danielsson, A.; Bhikhabhai, R.
Studies on the active site of deacetoxycephalosporin C synthase (1999), J. Mol. Biol., 287, 943-960.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.14.20.1	expression in Escherichia coli	Streptomyces clavuligerus

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
1.14.20.1	hanging drop method, recombinant enzyme expressed in Escherichia coli	Streptomyces clavuligerus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.14.20.1	0.0066	-	Penicillin N	-	Streptomyces clavuligerus
1.14.20.1	1.3	-	adipyl 6-aminopenicillanic acid	-	Streptomyces clavuligerus
1.14.20.1	2.03	-	penicillin G	-	Streptomyces clavuligerus

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
1.14.20.1	soluble	-	Streptomyces clavuligerus	-	-

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.14.20.1	34554	-	x * 34554, in solution the monomeric apoenzyme is in equilibrium with atrimeric form, electrospray mass spectrometry	Streptomyces clavuligerus

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.14.20.1	Streptomyces clavuligerus	P18548	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.14.20.1	efficient large-scale purification	Streptomyces clavuligerus
1.14.20.1	recombinant enzyme expressed in Escherichia coli	Streptomyces clavuligerus

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.14.20.1	66	-	recombinant enzyme	Streptomyces clavuligerus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.14.20.1	adipyl-6-aminopenicillanic acid + 2-oxoglutarate + O2	poor substrate	Streptomyces clavuligerus	7-adipylaminodesacetoxycephalosporanic acid + succinate + CO2 + H2O	-	?
1.14.20.1	penicillin G + 2-oxoglutarate + O2	-	Streptomyces clavuligerus	phenylacetyl-7-aminodeacetoxy cephalosporanic acid + succinate + CO2 + H2O	-	?
1.14.20.1	penicillin N + 2-oxoglutarate + O2	-	Streptomyces clavuligerus	deacetoxycephalosporin C + succinate + CO2 + H2O	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.14.20.1	?	x * 34554, in solution the monomeric apoenzyme is in equilibrium with atrimeric form, electrospray mass spectrometry	Streptomyces clavuligerus

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.14.20.1	0.062	-	penicillin G	-	Streptomyces clavuligerus
1.14.20.1	0.073	-	adipyl 6-aminopenicillanic acid	-	Streptomyces clavuligerus
1.14.20.1	0.42	-	Penicillin N	-	Streptomyces clavuligerus

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Release 2023.1 (January 2023)