Literature summary extracted from

Yeh, W.K.; Ghag, S.K.; Queener, S.W.
Enzymes for epimerization of isopenicillin N, ring expansion of penicillin N, and 3'-hydroxylation of deacetoxycephalosporin C. Function, evolution, refolding, and enzyme engineering (1992), Ann. N. Y. Acad. Sci., 672, 396-408.

No PubMed abstract available

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
5.1.1.17	expression in Penicillium chrysogenum	Streptomyces clavuligerus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
5.1.1.17	isopenicillin N	Streptomyces clavuligerus	-	penicillin N	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.14.20.1	Acremonium chrysogenum	-	bifunctional enzyme deacetoxycephalosporin C synthetase/hydroxylase	-
1.14.20.1	Streptomyces clavuligerus	-	-	-
5.1.1.17	Streptomyces clavuligerus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.14.20.1	purification of partially folded recombinant expandase/hydroxylase from insoluble granules of recombinant Escherichia coli	Acremonium chrysogenum

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.14.20.1	penicillin N + 2-oxoglutarate + O2	separate genes encode penicillin N expandase and DAOC hydroxylase	Streptomyces clavuligerus	deacetoxycephalosporin C + succinate + CO2 + H2O	-	?
1.14.20.1	penicillin N + 2-oxoglutarate + O2	bifunctional enzyme: penicillin N expandase/DAOC hydroxylase	Acremonium chrysogenum	deacetoxycephalosporin C + succinate + CO2 + H2O	-	?
5.1.1.17	isopenicillin	-	Streptomyces clavuligerus	penicillin N	-	?
5.1.1.17	isopenicillin N	-	Streptomyces clavuligerus	penicillin N	-	?

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Release 2023.1 (January 2023)