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Literature summary extracted from

  • Gao, S.; Von Schumann, G.; Stockigt, J.
    A newly-detected reductase from Rauvolfia closes a gap in the biosynthesis of the antiarrhythmic alkaloid ajmaline (2002), Planta Med., 68, 906-911.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.73 48000
-
 x * 48000, SDS-PAGE Rauvolfia serpentina

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.73 1,2-dihydrovomilenine + NADPH + H+ Rauvolfia serpentina enzyme is involved in the late stages of the biosynthesis of ajmaline 17-O-acetylnorajmaline + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.73 no activity in Catharanthus roseus
-
-
-
1.3.1.73 no activity in Gossypium hirsutum
-
-
-
1.3.1.73 no activity in Lonicera morrowii
-
-
-
1.3.1.73 no activity in Nicotiana tabacum
-
-
-
1.3.1.73 no activity in Saponaria officinalis
-
-
-
1.3.1.73 no activity in Solanum lycopersicum
-
-
-
1.3.1.73 Rauvolfia mannii
-
-
-
1.3.1.73 Rauvolfia mombasiana
-
-
-
1.3.1.73 Rauvolfia serpentina
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.73
-
 Rauvolfia serpentina

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.73 cell suspension culture
-
 Rauvolfia serpentina
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.73 additional information
-
-
 Rauvolfia serpentina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.73 1,2-dihydrovomilenine + NADPH + H+
-
 Rauvolfia serpentina 17-O-acetylnorajmaline + NADP+
-
 ?
1.3.1.73 1,2-dihydrovomilenine + NADPH + H+ enzyme is involved in the late stages of the biosynthesis of ajmaline Rauvolfia serpentina 17-O-acetylnorajmaline + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.73 ? x * 48000, SDS-PAGE Rauvolfia serpentina

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.73 37
-
-
 Rauvolfia serpentina

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.1.73 30 50 stable Rauvolfia serpentina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.73 6
-
-
 Rauvolfia serpentina

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.73 5.2 7.7 50% of maximal activity at pH 5.2 and at pH 7.7 Rauvolfia serpentina

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.3.1.73 5.8 6.2 in 50 mM citric acid, 100 mM Na2HPO4 buffer, stable Rauvolfia serpentina

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.73 NADPH
-
 Rauvolfia serpentina