BRENDA - Enzyme Database

Formation of (R)-configurated tetrahydroberberine alkaloids in vivo and in vitro

Bauer, W.; Zenk, M.H.; Tetrahedron Lett. 32, 487-490 (1991)
No PubMed abstract available

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.5.1.31
berberine + NADPH
Corydalis cava
(R)-tetrahydroberberines are formed from protoberberines by a highly stereoselective reduction system
canadine + NADP+
-
Corydalis cava
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.5.1.31
Corydalis cava
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.5.1.31
bulb
-
Corydalis cava
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.5.1.31
7,8-dihydroberberine + NADPH
at 64% of the activity with palmatine and NADPH
440357
Corydalis cava
canadine + NADP+
-
440357
Corydalis cava
r
1.5.1.31
berberine + NADH
at 59% of the activity with palmatine. At 15% of the activity with berberine and NADPH
440357
Corydalis cava
canadine + NAD+
-
440357
Corydalis cava
r
1.5.1.31
berberine + NADPH
at 59% of the activity with palmatine and NADPH
440357
Corydalis cava
canadine + NADP+
-
440357
Corydalis cava
r
1.5.1.31
berberine + NADPH
(R)-tetrahydroberberines are formed from protoberberines by a highly stereoselective reduction system
440357
Corydalis cava
canadine + NADP+
-
440357
Corydalis cava
r
1.5.1.31
columbamine + NADPH
at 39% of the activity with palmatine and NADPH
440357
Corydalis cava
? + NADP+
-
440357
Corydalis cava
r
1.5.1.31
coptisine + NADPH
at 47% of the activity with palmatine and NADPH
440357
Corydalis cava
? + NADP+
-
440357
Corydalis cava
r
1.5.1.31
dehydroscoulerine + NADPH
at 5% of the activity with palmatine and NADPH
440357
Corydalis cava
? + NADP+
-
-
-
r
1.5.1.31
dihydrosinactine + NADPH
at 52% of the activity with palmatine and NADPH
440357
Corydalis cava
? + NADP+
-
440357
Corydalis cava
r
1.5.1.31
jatrorrhizine + NADPH
at 25% of the activity with palmatine and NADPH
440357
Corydalis cava
? + NADP+
-
-
-
r
1.5.1.31
palmatine + NADPH
-
440357
Corydalis cava
? + NADP+
-
-
-
r
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.5.1.31
30
-
-
Corydalis cava
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.5.1.31
7.5
-
-
Corydalis cava
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.5.1.31
NADH
at 15% of the activity with NADPH
Corydalis cava
1.5.1.31
NADP+
-
Corydalis cava
1.5.1.31
NADPH
-
Corydalis cava
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.5.1.31
NADH
at 15% of the activity with NADPH
Corydalis cava
1.5.1.31
NADP+
-
Corydalis cava
1.5.1.31
NADPH
-
Corydalis cava
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.5.1.31
berberine + NADPH
Corydalis cava
(R)-tetrahydroberberines are formed from protoberberines by a highly stereoselective reduction system
canadine + NADP+
-
Corydalis cava
r
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.5.1.31
bulb
-
Corydalis cava
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.5.1.31
7,8-dihydroberberine + NADPH
at 64% of the activity with palmatine and NADPH
440357
Corydalis cava
canadine + NADP+
-
440357
Corydalis cava
r
1.5.1.31
berberine + NADH
at 59% of the activity with palmatine. At 15% of the activity with berberine and NADPH
440357
Corydalis cava
canadine + NAD+
-
440357
Corydalis cava
r
1.5.1.31
berberine + NADPH
at 59% of the activity with palmatine and NADPH
440357
Corydalis cava
canadine + NADP+
-
440357
Corydalis cava
r
1.5.1.31
berberine + NADPH
(R)-tetrahydroberberines are formed from protoberberines by a highly stereoselective reduction system
440357
Corydalis cava
canadine + NADP+
-
440357
Corydalis cava
r
1.5.1.31
columbamine + NADPH
at 39% of the activity with palmatine and NADPH
440357
Corydalis cava
? + NADP+
-
440357
Corydalis cava
r
1.5.1.31
coptisine + NADPH
at 47% of the activity with palmatine and NADPH
440357
Corydalis cava
? + NADP+
-
440357
Corydalis cava
r
1.5.1.31
dehydroscoulerine + NADPH
at 5% of the activity with palmatine and NADPH
440357
Corydalis cava
? + NADP+
-
-
-
r
1.5.1.31
dihydrosinactine + NADPH
at 52% of the activity with palmatine and NADPH
440357
Corydalis cava
? + NADP+
-
440357
Corydalis cava
r
1.5.1.31
jatrorrhizine + NADPH
at 25% of the activity with palmatine and NADPH
440357
Corydalis cava
? + NADP+
-
-
-
r
1.5.1.31
palmatine + NADPH
-
440357
Corydalis cava
? + NADP+
-
-
-
r
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.5.1.31
30
-
-
Corydalis cava
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.5.1.31
7.5
-
-
Corydalis cava