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Literature summary extracted from

  • Jiang, H.; Parales, R.E.; Gibson, D.T.
    The alpha subunit of toluene dioxygenase from Pseudomonas putida F1 can accept electrons from reduced FerredoxinTOL but is catalytically inactive in the absence of the beta subunit (1999), Appl. Environ. Microbiol., 65, 315-318.
    View publication on PubMedView publication on EuropePMC

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.11 Pseudomonas putida
-
 F1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.11 recombinant alpha-subunit of the oxygenase component Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.12.11 2.46
-
 units per mg of alpha-subunit of oxygenase Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.11 additional information the purified alpha-subunit of oxygenase component is reduced by NADH and catalytic amounts of reductaseTOL and ferredoxinTOL. Reduced alpha-subunit can not oxidize toluene and catalysis is strictly dependent on the presence of beta-subunit Pseudomonas putida ?
-
 ?
1.14.12.11 toluene + NADH + O2
-
 Pseudomonas putida (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.11 NADH
-
 Pseudomonas putida