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Literature summary extracted from

  • Lange, C.C.; Wackett, L.P.
    Oxidation of aliphatic olefins by toluene dioxygenase: enzyme rates and product identification (1997), J. Bacteriol., 179, 3858-3865.
    View publication on PubMedView publication on EuropePMC

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.11 Pseudomonas putida
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.11 (cis)-2-chloro-2-butene + NADH + O2 12% of the activity with toluene Pseudomonas putida 2-chloro-2-butene-1-ol + NAD+
-
 ?
1.14.12.11 (trans)-2-chloro-2-butene + NADH + O2 4% of the activity with toluene Pseudomonas putida ?
-
 ?
1.14.12.11 1,1-dichloro-1-propene + NADH + O2 18% of the activity with toluene Pseudomonas putida ?
-
 ?
1.14.12.11 1,1-dichloro-1-propene + NADH + O2 6% of the activity with toluene Pseudomonas putida 3,3-dichloro-2-propene-1-ol + NAD+
-
 ?
1.14.12.11 1-chloro-2-methyl-1-propene + NADH + O2 13% of the activity with toluene Pseudomonas putida 2-chloro-2-butene-1-ol + NAD+
-
 ?
1.14.12.11 2,3-dichloro-1-propene + NADH + O2 19% of the activity with toluene Pseudomonas putida 2,3-dichloro-2-propene-1-ol + NAD+
-
 ?
1.14.12.11 2-hexene + NADH + O2
-
 Pseudomonas putida hexane-2,3-diol + NAD+
-
 ?
1.14.12.11 3,4-dichloro-1-butene + NADH + O2 23% of the activity with toluene Pseudomonas putida 3,4-dichlorobutane-1,2-diol + NAD+
-
 ?
1.14.12.11 cis-1,2-dichloroethene + NADH + O2 12% of the activity with toluene Pseudomonas putida ?
-
 ?
1.14.12.11 cis-1,4-dichloro-2-butene + NADH + O2 18% of the activity with toluene Pseudomonas putida 1,4-dichlorobutane-2,3-diol
-
 ?
1.14.12.11 cis-1-bromo-1-propene + NADH + O2 11% of the activity with toluene Pseudomonas putida ?
-
 ?
1.14.12.11 cis-1-chloro-1-propene + NADH + O2 5% of the activity with toluene Pseudomonas putida ?
-
 ?
1.14.12.11 cis-2-heptene + NADH + O2
-
 Pseudomonas putida heptane-2,3-diol + NAD+
-
 ?
1.14.12.11 cis-2-octene + NADH + O2
-
 Pseudomonas putida octane-2,3-diol + NAD+
-
 ?
1.14.12.11 cis-2-pentene + NADH + O2 16% of the activity with toluene Pseudomonas putida pentane-2,3-diol + NAD+
-
 ?
1.14.12.11 cis-dibromoethene + NADH + O2 13% of the activity with toluene Pseudomonas putida 1,2-dibromoethane-1,2-diol + NAD+
-
 ?
1.14.12.11 additional information enzyme catalyzes monooxygenation and dioxygenation of aliphatic olefins Pseudomonas putida ?
-
 ?
1.14.12.11 toluene + NADH + O2
-
 Pseudomonas putida (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?
1.14.12.11 trans-1,4-dichloro-2-butene + NADH + O2 18% of the activity with toluene Pseudomonas putida 1,4-dichloro-2-butanone + NAD+
-
 ?
1.14.12.11 trans-1-bromo-1-propene + NADH + O2 3% of the activity with toluene Pseudomonas putida ?
-
 ?
1.14.12.11 trans-1-chloro-1-propene + NADH + O2 4% of the activity with toluene Pseudomonas putida ?
-
 ?
1.14.12.11 trans-dibromoethene + NADH + O2 5% of the activity with toluene Pseudomonas putida ?
-
 ?
1.14.12.11 trichloroethylene + O2 + NADH 25% of the activity with toluene Pseudomonas putida ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.11 NADH
-
 Pseudomonas putida