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Literature summary extracted from

  • Loke, P.; Sim, T.S.
    Site-directed mutagenesis of proline-285 to leucine in Cephalosporium acremonium isopenicillin N-synthase affects catalysis and increases soluble expression at higher temperatures (2001), Z. Naturforsch. C, 56, 413-415.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.21.3.1 expression of wild-type and mutant in Escherichia coli, evaluation of growth temperature for expression of the soluble mutant and wild-type in the Escherichia coli host BL21 (DE3) Acremonium chrysogenum

Protein Variants

EC Number Protein Variants Comment Organism
1.21.3.1 P285L site-directed mutagenesis, complete loss of activity, increased soluble expression in Escherichia coli host Acremonium chrysogenum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.3.1 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 Acremonium chrysogenum common step in the biosynthesis of penicillins, cephalosporins and cephamycins isopenicillin N + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.21.3.1 Acremonium chrysogenum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.3.1 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2
-
 Acremonium chrysogenum isopenicillin N + 2 H2O
-
 ?
1.21.3.1 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 common step in the biosynthesis of penicillins, cephalosporins and cephamycins Acremonium chrysogenum isopenicillin N + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.21.3.1 IPNS
-
 Acremonium chrysogenum
1.21.3.1 isopenicillin N-synthase
-
 Acremonium chrysogenum